吸烟大鼠脑血管内皮细胞P-选择素、E-选择素表达在脑血栓形成中的作用

目的观察吸烟大鼠脑动脉内皮细胞P-选择素、E-选择素表达,探讨吸烟致脑血栓形成的机制.方法建立吸烟动物模型,将50只大鼠随机分为正常对照组,短期大量吸烟组,长期小量吸烟组,长期大量吸烟组,戒烟组.免疫组织化学法检测大鼠脑动脉内皮细胞p-选择素、E-选择素和TNF-α的表达.结果吸烟大鼠脑血管内皮细胞P-选择素、E-选择素和TNF-α表达较不吸烟鼠明显增加(P＜0.05).长期大量吸烟组P-选择素、E-选择素和TNF-α表达比其他各组明显增多,戒烟组P-选择素、E-选择素和TNF-α表达下降,与其他各吸烟组相比有显著性差异(P＜0.05).结论吸烟致大鼠脑血管内皮细胞P-选择素、E-选择素和TNF-α表达升高是脑血栓形成的重要机制之一.

Effect of cigarette smoking on expression of P-selectin and E-selectin on cerebrovascular endothelial cells in rat''''s cerebral thrombosis

[Objective] To observe the expression of P-selectin and E-selectin on cerebrovascular endothelial cells of smoking rats in order to investigate pathogenesis of cerebral thrombosis induced by cigarette smoking.[Methods] Animal model of smoking rats were made, 50 male wistar rats were randomly grouped into normal control, short-term massive, long-term trifle, long-term massive and anti-smoking. Immunohistochemical technique was used to detect the expression of P-selectin, E-selectin and TNF-α in rat' s cerebrovascular endothelial cells. [Results] Compared with normal control, expression of P-selectin, E-selectin and TNF-α was increased notably in smoking groups (P ＜0.05). Expression of P-selectin, E-selectin and TNF-αin long-term massive smoking rats was significantly stronger than other groups. Expression of P-selectin, E-selectin and TNF-αwas decreased after antismoking and there were sifnificant differences compared with other groups (P ＜0.05). [Conclusion] Cigarette smoking increases expression of P-selectin, E-selectin and TNF-αin rat' s cerebrovascular endothelial cells, which may be one of pathogenesises in cerebral thrombosis.