参灵方对佐剂性关节炎大鼠胃黏膜损伤的保护机制研究

目的：研究探讨参灵方对佐剂性关节炎（ AA）大鼠胃黏膜损伤的保护作用机制。方法将60只Wistar大鼠随机抽取10只作为空白对照组，其余50只采用完全弗氏佐剂建立AA大鼠模型，造模成功后再随机抽取30只大鼠，分为模型对照组、雷尼替丁对照组及参灵方组，每组10只。雷尼替丁对照组组予盐酸雷尼替丁胶囊灌胃，参灵方组予参灵方灌胃，空白对照组及模型对照组分别予等容积的0．9％氯化钠注射液灌胃。各组大鼠均灌胃28 d后模型对照组、雷尼替丁对照组及参灵方组均腹部皮下注射吲哚美辛进一步诱导药源性胃黏膜损伤模型。光学显微镜观察胃黏膜组织病理变化，TUNEL法检测胃黏膜细胞凋亡情况。结果空白对照组大鼠胃黏膜结构层次清楚，上皮结构完整无损，腺体排列整齐；模型对照组大鼠胃黏膜上皮细胞明显受损，脱落，上皮结构不完整，部分黏膜中断，局部腺体结构紊乱，黏膜表面及腺腔内较多脱落的上皮细胞碎片及渗出物，胃黏膜细胞凋亡指数与空白对照组比较明显增加（P＜0．05）；雷尼替丁对照组及参灵方组大鼠胃黏膜局部上皮脱落较少，黏膜内血管充血，有少量炎细胞浸润，胃黏膜的细胞凋亡与模型组比较明显降低（P＜0．05），且组间比较差异无统计学意义（P＞0．05）。结论参灵方对AA大鼠胃黏膜损伤有明显保护作用，其可能是通过增加细胞增殖、减少细胞凋亡而抑制胃黏膜细胞损伤，从而发挥胃黏膜保护作用。

Study of protection mechanisms of Shenlingfang on gastric mucosal lesion in rats with adjuvant-induced ar-thritis

Objective To research of protection mechanisms of Shenlingfang on gastric mucosal lesion in rats with adjuvant -induced arthritis （ AA） .Methods 10 of 60 Wistar rats were randomly as control group , the rest rats were established AA model by complete Freund＆#39;s adjuvant .30 AA model rats were randomly divided into model group, ranitidine group and Shenlingfang group , 10 rats in each group .Rats in ranitidine group and Shenlingfang group separately received intragastric administration of ranitidine capsule solution and Shenlingfang .Rats in normal group and model group received intragastric administration of isometric distilled water for twenty -eight days .After 28 days, Rats in ranitidine group and Shenlingfang group received abdominal subcutaneous injection of indomethacin for further inducing drug -induced gastric mucosal injury model .The gastric mucosa pathological changes were observed by optical microscopy , apoptosis of gastric mucosa was measured by TUNEL assay .Results Gastric mucosa of rats in control group had clear hierarchy , intact epithelium structure and neatly arranged glands .Gastric mucosa of rats in model group had significantly impaired , shedding epithelial cells , incomplete epithelial structure , some broken muco-sa, local structural disorder glands , more debris and exudate of epithelial cells in mucous membranes surface and glandular cavity .Apoptotic index of gastric mucosa in model group was significantly increased as compared with that in control group （P〈0.05）.Gastric mucosa of rats in ranitidine group and Shenlingfang group had less shedding epithe -lial cells, mucosal vascular congestion , a small amount of inflammatory cell infiltration .Apoptotic index of gastric mu-cosa in ranitidine group and Shenlingfang group was significantly decreased as compared with that in model group （ P〈0.05）.The difference was not statistically significant between groups （P〉0.05）.Conclusion Shenlingfang has obvious protective effect on gastric mucosal lesion in rats with adjuvant-induced arthritis , it plays a role in gastric mucosal protection by increasing cell proliferation , reducing apoptosis＆amp;nbsp;and inhibiting gastric mucosal cell damage .