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Efficient recovery of fluoroquinolone-susceptible and fluoroquinolone-resistant Escherichia coli strains from frozen samples.
Authors:Ebbing Lautenbach  Evelyn Santana  Abby Lee  Pam Tolomeo  Nicole Black  Andrew Babson  Eli N Perencevich  Anthony D Harris  Catherine A Smith  Joel Maslow
Affiliation:Division of Infectious Diseases of the Department of Medicine, the Department of Biostatistics and Epidemiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6021, USA. ebbing@mail.med.upenn.edu
Abstract:
We assessed the rate of recovery of fluoroquinolone-resistant and fluoroquinolone-susceptible Escherichia coli isolates from culture of frozen perirectal swab samples compared with the results for culture of the same specimen before freezing. Recovery rates for these 2 classes of E. coli were 91% and 83%, respectively. The majority of distinct strains recovered from the initial sample were also recovered from the frozen sample. The strains that were not recovered were typically present only in low numbers in the initial sample. These findings emphasize the utility of frozen surveillance samples.
Keywords:
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