宁夏抗病毒治疗患者HIV基因型耐药及亚型分析

HIV drug resistance and subtypes in HIV-infected patients receiving antiretroviral treatment,Ningxia

Objective To probe into the prevalence of HIV(HIV)resistant strains and distribution of subtypes in patients receiving highly active antiretroviral therapy(HAART)in Ningxia. Methods A cross-sectional survey was conducted among the HIV/AIDS patients with HAART for more than 6 months in Ningxia,2011.The blood samples were collected,and the HIV-1 viral load was tested.Drug resistance genotypes were detected for the samples with viral loads higher than 1000 copies/ml. Results A total of 631 HIV/AIDS patients were investigated.The rate of the virus uninhibition was 10.3%.57 gene sequences acquired were used for genotypic resistance analysis,in which 29 cases had drug resistance mutation.The incidence of drug resistance of people with virus uninhibition was 50.88%,The drug resistance rate of people surveyed was 4.65%.Cases resistant to NRTIs was 20.27 cases were resistant to NNRTIs,and the other one was resistant to PIs.The main mutation site of NNRTIs was M184V/M184I,and the main mutation sites of NRTIs were K103N,Y181C/Y181I 8,G190S /G190A,PIs mutations in major resistance sites and secondary resistance mutations were M46I and Q58E.The predominant subtype of the resistant mutant were CRF01_AE and CRF07_BC subtype. Conclusion The rates of drug resistance of HIV/AIDS patients is low in Ningxia province.The predominant subtype of the resistant mutant is CRF01_AE and CRF07_BC subtype.Enhanced supervision measures should be taken to prevent the spread of the resistant mutant.