小陷胸汤加味方预处理对兔心肌缺血再灌注损伤血清MDA、SOD的影响

目的观察小陷胸汤加味方预处理对兔心肌缺血再灌注损伤的保护作用。方法将32只健康日本大耳白兔随机分为假手术组、模型组和小陷胸汤加味方低、高剂量组各8只。结扎左冠状动脉前降支30min再灌注120min后立即右心房取血,分离血清,观察血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果与假手术组比较,模型组和小陷胸汤加味方低、高剂量组兔血清的SOD、MDA比较差异均有统计学意义(P0.05);与模型组比较,小陷胸汤加味方低剂量组与小陷胸汤加味方高剂量组兔血清的SOD、MDA比较差异均有统计学意义(P0.05);小陷胸汤加味方低剂量组与小陷胸汤加味方高剂量组兔血清SOD、MDA比较差异无统计学意义(P0.05)。结论小陷胸汤加味方预处理对兔心肌缺血再灌注损伤具有保护作用,其机制可能与清除自由基有关。

Effect of Xiaoxianxiong decoction on MDA and SOD in serum of rabbit with myocardial ischemia reperfusion injury

Objective To observe the protective effect of additional Xiaoxianxiong decoction pretreatment in rabbits with myocardial ischemia reperfusion injury （MIRI）. Methods Totally 32 rabbits, with health, of clean grade, of male, were divided randomly into 4 groups（ n = 8 in each group） ： sham operation group （A group）, model group （B group）, traditional Chinese medicine low dosage group （C group） and high dosage group （D group）. The levels of serum SOD and MDA content were measured. Results After ischemia reperfusion, serum SOD and MDA content in B, C and D group were significantly different as compared with A group （ P 〈 0.05 ） ; serum SOD and MDA content in C and D group were significantly different as compared with B group （ P 〈 0.05 ） ; The serum SOD and MDA content in C group were not significantly different as compared with D group （ P 〉 0. 05 ）. Conclusion Additional Xiaoxianxiong decoction has the function of cleaning out oxygen free radical, alleviating the extent of the myocardial ischemia reperfusion injury.