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| 冬凌草甲素对鼻咽癌HNE-1细胞株生长及其PCNA、Caspase-3表达的影响 | |
| 引用本文： | 向银洲,余林,魏莲枝,贵平,彭平,熊志洁. 冬凌草甲素对鼻咽癌HNE-1细胞株生长及其PCNA、Caspase-3表达的影响[J]. 山东医药, 2010, 50(50) |
| 作者姓名： | 向银洲 余林 魏莲枝 贵平 彭平 熊志洁 |
| 作者单位： | 宜昌市第一人民医院 重庆医科大学附属第一医院 |
| 摘 要： | 目的观察冬凌草甲素对人鼻咽癌细胞株HNE-1生长及其细胞核抗原(PCNA)、Caspase-3表达的影响。方法 HNE-1中加入冬凌草甲素,MTT法观察其生长情况,FCM检测细胞周期,免疫细胞化学染色法检测PCNA,Western-blot技术检测Caspase-3。结果加入冬凌草甲素后HNE-1细胞生长受抑制,细胞被阻滞于G_0/G_1期;冬凌草甲素作用48 h后,HNE-1细胞的PCNA蛋白表达下调,Caspase-3蛋白表达增加。结论冬凌草甲素在体外可显著抑制人鼻咽癌细胞株HNE-1细胞生长并诱导其凋亡,其机制可能是下调PCNA蛋白表达,激活Caspase-3的活性。 |
| 关 键 词： | 冬凌草甲素 鼻咽癌 HNE-1细胞株 细胞核抗原 半胱氨酸蛋白酶 |
Effects of oridonin on growth and PCNA,Caspase-3 expression of human nasopharyngeal carcinoma HNE-1 cell lines |
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| XIANG Yin-zhou,YU Lin,WEI Lian-zhi,GUI Ping,PENG Ping,XIONG Zhi-Jie. Effects of oridonin on growth and PCNA,Caspase-3 expression of human nasopharyngeal carcinoma HNE-1 cell lines[J]. Shandong Medical Journal, 2010, 50(50) | |
| Authors: | XIANG Yin-zhou YU Lin WEI Lian-zhi GUI Ping PENG Ping XIONG Zhi-Jie |
| Affiliation: | 1 The First Hospital of Yichang; Yichang 443000; P.R.China); |
| Abstract: | Objective To observe the effect of oridonin on growth of human nasopharyngeal carcinoma HNE-1 cell lines and its effect on proliferation cell nuclear antigen(PCNA) and Caspase-3 expression.Methods HNE-1 was cultured with oridonin.Growth of HNE-1 cells was observed by MTT assay.Cell cycle was measured by Flow cytometry(FCM.). The expression of PCNA was assessed by immunocytochemistry method.The protein expression of Caspase-3 was examined by Western-blot.Results The growth of HNE-1 cells were inhibited by or... |
| Keywords: | oridonin nasopharyngeal carcinoma HNE-1 cell lines proliferation cell nuclear antigen cysteine-containing aspartate-specific proteases |
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