柚皮素预处理对大鼠心肌缺血再灌注损伤的保护作用

目的观察柚皮素（naringenin，NAR）对大鼠心肌缺血再灌注损伤（IRI）的保护作用。方法32只雄性SD大鼠（220 g~250 g）随机分为假手术组（control，n=8）、IRI组（n=8）、NAR 50 mg/kg组（n=8）和NAR 100 mg/kg组（n=8）。NAR 50 mg/kg和NAR 100mg/kg组均在IRI前2 h予相应NAR腹腔注射。大鼠心肌IRI模型制备方法：结扎左冠状动脉前降支30 min，再灌注180 min，而后180 min检测各组大鼠血清白介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）、乳酸脱氢酶（LDH）、肌酸激酶（CK）、超氧化物歧化酶（SOD）和丙二醛（MDA）含量以及再灌后24 h检测心肌梗死面积。结果NAR可显著降低大鼠心肌IRI后心肌梗死面积（与IRI相比，P＜0.05），同时还可以显著减轻IRI后血清中IL-1β、TNF-α、LDH、CK和MDA的水平，增加SOD水平（与IRI相比，P＜0.05），并呈浓度依赖性。结论NAR对心肌IRI具有明显的保护作用，其保护机制与抑制IRI后炎症反应和清除氧化应激损伤有关。

Effect of naringenin on myocardial ischemia-reperfusion injury in rats

Objective To observe the effect of naringenin （NAR） on myocardial ischemia-reperfusion injury （IRI） in rats. Methods Male SD rats （weighted 220 g to 250 g, n=32） were randomly divided into control group, IRI group, NAR group 1 （50 mg/kg） and NAR group 2 （100 mg/kg, each n=8）. NAR group 1 and NAR group 2 were intraperitoneally injected NAR two hours before IRI. The model of IRI was established as follows：ligating the left anterior descending coronary artery for 30 minutes and then giving reperfusion for 180 minutes. After three hours, the levels of serum interleukin 1β（IL-1β）, tumor necrosis factor-α（TNF-α）, lactic dehydrogenase （LDH）, creatine kinase （CK）, superoxide dismutase （SOD） and malondialdehyde （MDA）, and infarction area after reperfusion for 24 hours were detected in all groups. Results Compared with control group, the infarction area was significantly reduced and the levels of serum IL-1β, TNF-α, LDH, CK and MDA decreased in NAR groups （P〈0.05）, and SOD level （P〈0.05） increased showing concentration dependent. Conclusion NAR has a significant effect on myocardial IRI, and the mechanism may be related to inhibiting inflammatory reaction after IRI and relieving oxidative stress injury.