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Pyrosequencing-based approach for rapid detection of rifampin-resistant Mycobacterium tuberculosis
Authors:Zhao Jin Rong  Bai Yu Jie  Zhang Qing Hua  Wang Yan  Luo Ming  Yan Xiao Jun
Affiliation:Institute of Genetic Diagnosis, Fourth Military Medical University, Xi'an 710032, People's Republic of China. zhaojrr@126.com
Abstract:Rifampin resistance in Mycobacterium tuberculosis could be determined within 2 h by using pyrosequencing-based approach. Pyrosequencing results of rifampin-resistant (n = 21) and rifampin-susceptible (n = 20) M. tuberculosis isolates were concordant with those based on drug susceptibility testing and conventional DNA sequencing. Results showed pyrosequencing-based approach is a rapid, sensitive, and efficient detection method of rifampin-resistant M. tuberculosis.
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