弓形虫眼病小鼠模型的建立及其眼底特征

目的 建立弓形虫眼病小鼠模型, 观察其眼底改变和病理特征。方法 将20只BALB/C小鼠随机分为对照组10只和实验组10只, 实验组小鼠经口给予Ⅱ型弓形虫PRU虫株(弱毒株)包囊, 模拟弓形虫慢性感染过程, 每天观察小鼠眼底情况, 在1、2、3个月后分别给两组小鼠做眼底照相, 3个月后ELISA法检测小鼠血清IgG, 然后处死小鼠取眼球做石蜡切片, HE染色后观察视网膜及脉络膜损害情况。结果 实验组小鼠2周后即可观察到眼底出现明显炎症表现, 视盘边界不清, 视网膜血管充盈, 视网膜水肿、出血, 由视盘区开始逐渐向周边血管扩展, 并随时间延长逐渐加重, 血清IgG出现明显高滴度变化, 显著高于对照组(P<0.001)。视网膜及脉络膜组织切片, 可见视网膜及脉络膜明显出现水肿改变, 组织间隙明显疏松, 有腔隙形成, 视网膜及脉络膜组织可见炎症细胞浸润。结论 通过经口给予Ⅱ型弓形虫PRU虫株可以建立小鼠弓形虫眼病模型, 证明获得性弓形虫病也可以引起脉络膜视网膜的炎症表现, 为弓形虫眼病的深入研究奠定基础。

Murine model of ocular toxoplasmosis and feature of fundus

Objective To establish murine model for ocular toxoplasmosis and observe the fundus and pathological changes. Methods 20 BALB/C mice were randomly divided into the control and experimental group with 10 mice per group. In the experimental group, mice were fed orally with toxoplasma type Ⅱ PRU strains cyst. Changes of fundus were observed daily and fundus photography were taken after 1 month, 2 months and 3 months, respectively. After 3 months, serum IgG of the mice was detected by ELISA assays. The mice were sacrificed and the eyes were harvested. Pathological damages of retina and choroid tissue were observed after HE staining. Results After two weeks, fundus inflammation was noticed in the experimental group, including optic disc edema, retinal angioplerosis, edema and hemorrhage along with retinal vascular, from the beginning of the optic disc area gradually expanded to the surrounding blood vessels. These changes aggravated with time. Significant titers change in serum IgG, significant higher than the control group (P<0.001), from the retinal and choroid tissue slice of the experimental group mice, we could see the retinal and choroid apparent edema, the gap between the organization was loose, lacuna was formed,inflammatory cell infiltration was found in tissue of retinal and choroid. Conclusion Infecting mice with type Ⅱ toxoplasma PRU strains via oral route could be a good way to establish animal model for ocular toxoplasmosis, which can cause inflammation of the choroid and retina.