红斑狼疮皮损中CD4^＋、CD8^＋T细胞表达及其与Bcl-2关系的研究

目的：确定Bcl-2及CD4^＋、CD8^＋T细胞在红斑狼疮皮损区的表达并探讨其与红斑狼疮发病的关系。方法：采用免疫组化法对30例红斑狼疮皮损区T细胞亚群CD4^＋、CD8^＋T细胞及淋巴细胞Bcl-2进行检测，并与17例正常皮肤作对照。结果：红斑狼疮患者皮损中Bcl-2淋巴细胞和CD4^＋T细胞的表达明显高于正常对照组，而CD8^＋T细胞表达明显低于正常对照组；Bcl-2的表达与CD4^＋T细胞表达呈正相关，与CD8^＋T细胞表达呈负相关。结论：红斑狼疮皮损区淋巴细胞Bcl-2的表达增强可能会导致CD4^＋和CD8^＋T细胞凋亡异常，从而导致免疫紊乱而发病。

Expression of CD4 ^＋/CD8 ^＋ in the lesions of lupus erythematosus and its relationship with Bcl- 2

Objective： To investigate the expression of Bcl - 2 and CD4^ ＋/CD8 ^＋ in the lesions of lupus erythematosus （LE） and to correlate the relationship between their expression and the pathogenesis of LE. Methods： The expression of Bcl - 2 and CD4^＋/CD8^＋ in the skin lesions of 30 patients with LE was tested with immunohistochemistry and 17 normal specimens were taken as controls. Results： While the expression Bcl- 2 and CD4^＋ was higher in LE than in controls, CD8 ^＋ was lower in LE than in control. There was a positive correlation between the expression of Bcl - 2 and CD4^ ＋ T cells and the expression of Bcl - 2 was negatively correlated with the expression of CD8 ^＋ T cells. Conclusion： The higher expression of Bcl - 2 may lead to abnormal apoptosis of CD4^ ^＋ and CD8 ＋ cells and immunity disorder in LE.