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| 戊四氮点燃模型大鼠海马苔藓纤维出芽与Cdk5/p35表达的动态变化 | |
| 引用本文： | 田发发,郭婷辉,陈金梅,马云峰,曾畅,李凤容,王新华,傅晶晶. 戊四氮点燃模型大鼠海马苔藓纤维出芽与Cdk5/p35表达的动态变化[J]. 中南大学学报(医学版), 2008, 33(12): 1101-1107 |
| 作者姓名： | 田发发 郭婷辉 陈金梅 马云峰 曾畅 李凤容 王新华 傅晶晶 |
| 作者单位： | 1．中南大学湘雅医院神经内科，长沙410008；2.福建医科大学附属漳州市 医院神经内科，福建漳州363000；3.上海市普陀区人民医院神经内科， 上海200060；4.北京市三博复兴脑科医院，北京100038 |
| 基金项目： | 湖南省教育厅资助项目 |
| 摘 要： | 目的：动态观察细胞周期素依赖性激酶5（cyclin-dependent kinase5，Cdk5）和调节亚基p35在戊四氮（pentylenetetrazole，PTZ）致痫大鼠海马各区的表达变化，及其与苔藓纤维出芽（mossy fiber sprouting，MFS）的关系，以探讨其在癫痫发病机制中的作用。方法：SD雄性成年大鼠120只，随机分为PTZ组和对照组；PTZ组分为PTZ第1次注射后3d、1周、2周、4周、6周共5个亚组，每亚组12只，对照组随机分为5个亚组，与眦组各时间点对应。以上各亚组再分2个小组，每小组6只大鼠，分别进行（1）免疫组织化学和原位杂交，检测各时间点Cdk5与p35蛋白和mRNA在海马CA1和CA3区、齿状回颗粒细胞层和门区的表达；（2）Timm染色并评分。结果：PTZ组大鼠上述各区Cdk5和p35mRNA在第3天时表达即有明显上调（P＜0．01），第4周仍维持在较高水平，第6周时基本恢复到对照组水平；各区Cdk5和p35蛋白表达从第3天起即上调（P＜0．01），第2周时最强，其后下调，至第6周接近或达到对照组水平。PTZ组CA3区在点燃前MFS评分1～4分，点燃后MFS评分4～5分。MFS的变化趋势与Cdk5和p35的表达一致。结论：海马Cdk5／p35的表达变化可能参与苔藓纤维出芽，从而促进癫痫的发生。 |
| 关 键 词： | 戊四氮 点燃模型 细胞周期素依赖性激酶 苔藓纤维出芽 |
| 收稿时间： | 2008-05-05 |
Hippocampal mossy fiber sprouting and Cdk5/p35 expressions in the pentylenetetrazole kindling rat model |
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| TIAN Fa-fa,GUO Ting-hui,CHEN Jin-mei,MA Yun-feng,ZENG Chang,LI Feng-rong,WANG Xin-hua,FU Jing-jing. Hippocampal mossy fiber sprouting and Cdk5/p35 expressions in the pentylenetetrazole kindling rat model[J]. Journal of Central South University. Medical sciences, 2008, 33(12): 1101-1107 | |
| Authors: | TIAN Fa-fa GUO Ting-hui CHEN Jin-mei MA Yun-feng ZENG Chang LI Feng-rong WANG Xin-hua FU Jing-jing |
| Affiliation: | 1. Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008；2. Department of Neurology, Zhangzhou Hospital,Fujian Medical University, Zhangzhou Fujian 363000；3. Department of Neurology, Putuo Hospital in Shanghai,Shanghai 200060；4. Department of Neurology, Sanbofuxing Brain Hospital, Beijing 100038,China |
| Abstract: | Objective To observe the expression of cyclin-dependent kinase 5(Cdk5) and p35 in rats' hippocampus during pentetrazole kindling process and their relation with mossy fiber sprouting(MFS),and to investigate the role of Cdk5/p35 in epileptogenesis.MethodsAltogether 120 healthy male SD rats were randomly divided into a control group and a pentylenetetrazole(PTZ) treated group.The epileptic models were established by the injection of PTZ intraperitoneally while the control rats were injected with an equal dose... |
| Keywords: | pentylenetetrazole kindling model cyclin-dependent kinase 5 mossy fiber sprouting |
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