RP-HPLC法测定不同品种西番莲叶中异荭草素、荭草素含量

目的：采用高效液相色谱法测定不同品种西番莲叶中异荭草素、荭草素的含量。方法：采用ODS—C18色谱柱（250mm×4．6mm，5μm），流动相为水-四氢呋喃-并丙醇·乙腈（87：8：2：3），检查波长为360nm，柱温30℃，流速1ml·min^-1。结果：异荭草素、荭草素线性范围分别为0．0367～0．5872mg·ml^-1（r=0．9998）、0．0372～0．5955mg·ml^-1（r=0．9995），平均回收率分别为98．88％（n=9）、98．84％（n=9）。结论：不同品种西番莲叶中异荭草素、荭草素含量有明显差异，可为西番莲叶质量评价提供参考。

Determination of Isoorientin and Orientin of Passiflora Leaves in Various Specimens by RP-HPLC

Objective： To determine the contents of isoorientin and orientin of passiflora leaves in various specimens by RP-HPLC. Method： ODS C,s column （250mm × 4.6mm,5 μm） was used , the mobile phase consisted of a quaternary-tetrahydrofuran-isopropanolacetonitrile（87： 8： 2： 3, V/V）, the flow-rate was 1.0ml· min^-1, the temperature of column was 30℃ and detection wavelength was at 360nm. Result： The good linearities of isoorientin and orientin were obtained within the ranges of 0.0367 - 0.587mg· ml^ - 1 （ r = 0.999 8 ） and0.0372 - 0. 5955mg· m1 ^-1 （ r = 0. 999 5 ）, the average recoveries were 98.88 % （ n = 9 ） and 98.84% （ n = 9 ）, respectively. Conclusion： The content of isoorientin and oricntin in Passiflora Leaves from various specimens are different. It provides a reference standard for the quality control of passiflora leaves.