| 不同发育阶段大鼠脑髓鞘化的研究 |
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| 引用本文： | 张雨平,黄其林,赵聪敏,汤金梁,王亚丽. 不同发育阶段大鼠脑髓鞘化的研究[J]. 第三军医大学学报, 2009, 31(22): 2189-2192 |
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| 作者姓名： | 张雨平 黄其林 赵聪敏 汤金梁 王亚丽 |
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| 作者单位： | 第三军医大学新桥医院儿科,重庆,480037;第三军医大学新桥医院神经外科,全军癫痫病诊治中心,重庆,480037;第三军医大学新桥医院神经外科,全军癫痫病诊治中心,重庆,480037;第三军医大学新桥医院儿科,重庆,480037;第三军医大学新桥医院病理科,重庆,480037 |
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| 基金项目： | 国家计生委科技攻关项目 |
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| 摘 要： | 目的 研究大鼠不同发育阶段脑髓鞘化的发展及规律.方法 应用LFB髓鞘染色、MBP免疫组化及Wes-tern blot观察不同发育阶段大鼠脑髓鞘的分布及变化.结果 LFB及MBP免疫组化染色E18、P0、P2均未见着色,P7大鼠胼胝体中部少量LFB着色,P15两种染色均呈阳性,P30着色加深,与P90、P720类似.通过Western blot检测MBP蛋白在孕中后期已有表达,出生后随日龄增长逐渐增加,至老年期表达量最高.结论 大鼠脑髓鞘化从P15已经开始,P30接近成熟;观察髓鞘的最佳时间在30 d以后.
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| 关 键 词： | 大鼠 脑 髓鞘化 发育 |
Myelination of rat brain at different developmental stages |
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| ZHANG Yu-ping,HUANG Qi-lin,ZHAO Cong-min,TANG JIN-liang,WANG Ya-li. Myelination of rat brain at different developmental stages[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(22): 2189-2192 |
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| Authors: | ZHANG Yu-ping HUANG Qi-lin ZHAO Cong-min TANG JIN-liang WANG Ya-li |
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| Affiliation: | ZHANG Yu-ping1,2,HUANG Qi-lin2,ZHAO Cong-min1,TANG JIN-liang3,WANG Ya-li3(1Department of Paediatrics,2Department of Neurosurgery,3Department of Pathology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China) |
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| Abstract: | Objective To investigate the myelination of rat brain at different developmental stages.Methods Luxol fast blue staining,immunohistochemistry and Western blotting were applied to determine the distribution and alternation of myelin sheath in the brains of SD rats at different developmental stages.Results Negative LFB and MBP staining were shown at the stages of embryonicage 18 d(E18),postnatal age 0 d(P0) and P2.At P7,the corpus callosum was weakly stained by LFB.The positive stain of LFB and MBP occurred i... |
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| Keywords: | rat brain myelination development |
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