| 苯乙醇苷类化合物与ctDNA的相互作用 |
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| 引用本文： | 袁欢,王涛,祝晨蔯,吴爱芝,林朝展.苯乙醇苷类化合物与ctDNA的相互作用[J].中国中药杂志,2017,42(14):2725-2729. |
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| 作者姓名： | 袁欢 王涛 祝晨蔯 吴爱芝 林朝展 |
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| 作者单位： | 广州中医药大学中 药学院, 广东 广州 510006,广州中医药大学中 药学院, 广东 广州 510006,广州中医药大学 临床药理研究所, 广东 广州 510405,广州中医药大学中 药学院, 广东 广州 510006,广州中医药大学 临床药理研究所, 广东 广州 510405 |
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| 基金项目： | 江苏高校优势学科建设工程项目（YSXK-2014）；江苏高校品牌专业建设工程项目（PPZY2015A070） |
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| 摘 要： | 以中性红为分子探针研究2种苯乙醇苷类化合物(PhGs)毛蕊花糖苷和连翘酯苷B在生理条件下与小牛胸腺DNA(ctDNA)之间的相互作用。荧光光谱、紫外光谱、黏度、DNA热变性实验和分子对接结果表明:这2种PhGs主要采用氢键作用以沟区方式结合到ctDNA双螺旋小沟中,毛蕊花糖苷与DNA结合作用更强。
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| 关 键 词： | 苯乙醇苷 ctDNA 中性红 分子对接 |
| 收稿时间： | 2017/3/25 0:00:00 |
Interaction of phenylethanoid glycosides and calf thymus DNA |
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| YUAN Huan,WANG Tao,ZHU Chen-chen,WU Ai-zhi and LIN Chao-zhan.Interaction of phenylethanoid glycosides and calf thymus DNA[J].China Journal of Chinese Materia Medica,2017,42(14):2725-2729. |
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| Authors: | YUAN Huan WANG Tao ZHU Chen-chen WU Ai-zhi and LIN Chao-zhan |
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| Institution: | School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China,School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China,Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China,School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China and Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China |
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| Abstract: | In physiological condition, the interaction of acteoside and forsythoside B with calf thymus DNA using neutral red (NR) as a fluorescence probe were investigated by fluorescence, UV-visible spectrophotometry, viscosity, DNA melting techniques, and molecular docking. It is observed that acteoside and forsythoside B can react with DNA. The major mode of recognition between drug and DNA is groove binding by hydrogen bonds, and the interaction of acteoside with DNA is stronger than that of forsythoside B. |
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| Keywords: | phenylethanoid glycosides ctDNA neutral red molecular docking |
| 本文献已被 CNKI 等数据库收录！ |
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