新生儿病房进行肠道病毒筛查的意义

目的 探讨新生儿病房患儿入院时即进行肠道病毒筛查的意义.方法 在2009年5月～2010年5月对每位新人患儿首先进行隔离,然后进行咽拭子及粪便采样,采样用RT-PCR方法检测肠道病毒通用型、肠道病毒71型、柯萨奇A16型.对采样检测阳性的个体及密切接触人群进行系统的流行病学研究.结果 新生儿存在肠道病毒宫内感染、产时感染和生后感染几种方式,感染率分别为2.72%、6.28%、15.76%.结论 预防新生儿肠道病毒感染在院前及院内同样重要.

Clinical significance of neonatal screening for enterovirus

Objective To explorer the significance of screening for enterovirus when newborn patients hospitalized in neonatal room. Methods All cases were collected in neonatal room from May, 2009 to May, 2010. Every newborn patient was isolated and collected specimens (including stool and throat swabs). These clinical specimens were directly tested by reverse transcriptase polymerase chain reaction ( RT - PCR) assay with universal enterovirus, CA16 - specific and EV71 - specific primers. The epidemiologic studies on enterovirus for EV PCR -positive individuals and their close contacts were carried out. Results The modes of transmission of neonatals enteroviral infections were: intrauterine infection, intrapartum infection, and puerperal infection and their infection rates were 2.72% , 6.285, 15.76% respectively. Conclusions Screening in all newborns for preventing enterovirus infection plays equally important role in pre - hospitalization and during hospitalization.