不同病程糖尿病大鼠肾脏一氧化氮变化与氧自由基的关系

为探讨糖尿病（ＤＭ）大鼠肾脏一氧化氮（ＮＯ）变化与氧自由基的关系，测定链脲佐菌素（ＳＴＺ）诱导的ＤＭ大鼠及对照组（ＮＣ）于病程２，８，１６周时肾组织ＮＯ含量一氧化氮合酶（ＮＯＳ）活性，指质过氧化物（ＬＯＰ）水平及超氧化物歧化酶（ＳＯＤ）、过氧化物酶（ＰＯＤ）和过氧化氢酶（ＣＡＴ）活生变化。结果表明：ＤＭ大鼠肾脏ＮＯ水平及ＮＯＳ活性随病程的延长由升高至下降；各病程ＤＭ大鼠肾脏ＬＰＯ水平均高于同批ＮＣ

Relationship between nitric oxide and oxygen free radicals in different duration of diabetes in rat kidney]

To study the relationship between nitric oxide(NO) and oxygen free radicals in rat kidney of diabetes mellitus(DM), the authors examined the changes of renal tissue NO level and nitric oxide synthase(NOS) activity, lipid peroxidation(LPO) level and superoxide dismutase(SOD), peroxidase(POD) and catalase (CAT) activities in streptozotocin(STZ)-induced diabetic rats and normal controls(NC) at the 2nd, 8th- and 16th-week. The results showed that renal-tissue NO level and NOS activity were increased at the 2nd week, but decreased at the 8th- and 16th-week; LPO levels were significantly elevated in all DM groups than those in NC groups. SOD, POD and CAT activities decreased significantly in the 8th and 16th week DM groups compared with those in NC groups. The results suggest that NO level in kidney of STZ-induced diabetic rats dynamically change with the disease course of development. This change may be related with the change of anti-oxidation in kidney which high glucose induces.