| 百日咳菌液对大鼠培养脑切片兴奋性氨基酸释放的影响 |
| |
| 引用本文： | 尹飞,杨于嘉.百日咳菌液对大鼠培养脑切片兴奋性氨基酸释放的影响[J].湖南医科大学学报,1999,24(6):510-512. |
| |
| 作者姓名： | 尹飞 杨于嘉 |
| |
| 摘 要： | 目的：了解百日咳菌液对培养的大鼠脑切片兴奋性氨基酸（ＥＡＡｓ）释放的影响及１１双氢－５Ｈ－二苯（ａ，ｂ）－环庚烯亚胺（ＭＫ－８０１）对脑切片有无保护作用，方法：ＳＤ大鼠随机分成正常脑切片培养组（ＮＣ组）１０％百日咳菌液脑切片培养组（ＰＢＣ组）和ＭＫ－８０１预处理后１０％百日咳菌液脑切片培养组（ＭＰＢＣ组）及乳酸脱相色谱法测脑切片培养上清液中的ＥＡＡｓ释放量，结果：ＰＢＣ组的谷氨酸（Ｇｌｕ）门冬氨酸
|
| 关 键 词： | 百日咳杆菌 氨基酸 MK－801 大鼠 脑水肿 |
Effect of pertussis bacilli on the release of excitatory amino acids in brain slice of rats] |
| |
| F Yin,Y Yang,P Yu.Effect of pertussis bacilli on the release of excitatory amino acids in brain slice of rats][J].Bulletin of Hunan Medical University,1999,24(6):510-512. |
| |
| Authors: | F Yin Y Yang P Yu |
| |
| Institution: | Department of Pediatrics, Xiangya Hospital, Hunan Medical University, Changsha 410008. |
| |
| Abstract: | OBJECTIVE: To investigate the effect of pertussis bacilli on the release of excitatory amino acids (EAAs) in rat-brain slice and to know whether MK-801(dizocipine) protects brain tissue from excitotoxicity. METHODS: SD rats were randomly divided into 3 groups: 1. Normal slice cultured group(NC); 2. 10% pertussis bacilli(PB) slice cultured group(PBC); 3. 10% PB slice cultured group after pretreatment with MK-801. The release of EAAs from brain slices was measured by high performance liquid chromatography. RESULTS: The concentrations of glutamate(Glu 29.53 +/- 8.00), aspartate(Asp 23.74 +/- 5.79) and LDH(38.03 +/- 8.45) were significantly higher in PBC group than those(Glu 16.46 +/- 4.00, Asp 15.27 +/- 5.31, LDH 23.10 +/- 5.11) in MPBC group and (Glu 16.09 +/- 3.74, Asp 11.47 +/- 2.51, LDH 25.82 +/- 4.50) NC group. CONCLUSION: Pertussis bacilli may elicit a large increase of extracellular EAAs and LDH in vitro, while MK-801 protects brain tissue from excitotoxicity. |
| |
| Keywords: | |
| 本文献已被 维普 等数据库收录！ |
|
