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| 聚合酶链反应检测细菌16SrRNA基因 | |
| 引用本文： | 李聪智 谭德明. 聚合酶链反应检测细菌16SrRNA基因[J]. 湖南医科大学学报, 1999, 24(2): 136-138 |
| 作者姓名： | 李聪智 谭德明 |
| 摘 要： | 根据细菌１６ＳｒＲＮＡ基因的高度保守性，设计合成所有细菌，革兰氏阳性细菌及革兰氏阴性细菌的共同引物，采用聚合酶链反应检测已知细菌１３株，三对引物分别扩增的阳性率为１００％，倍比稀释法能检出细菌浓度为４ＣＦＵ．ｍｌ＾－１，同时检测临床样本４０份，阳性率为６７．５％，同期细菌培养阳性率为４５％，二者比较差异有显著性。 |
| 关 键 词： | 聚合酶链反应 细菌 16SrRNA基因 |
PCR for the detection of 16S rRNA gene bacteria] |
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| C Li,D Tan,M Lu,Y Wu,W Zhou,G Liu,A Liu. PCR for the detection of 16S rRNA gene bacteria][J]. Bulletin of Hunan Medical University, 1999, 24(2): 136-138 | |
| Authors: | C Li D Tan M Lu Y Wu W Zhou G Liu A Liu |
| Affiliation: | Department of Infection, Xianya Hospital, Hunan Medical University, Changsha 410008. |
| Abstract: | According to the high conservative region of 16S rRNA gene in bacteria, PCR primers of the broad-range bacteria, gram-positive bacteria and gram-negative bacteria were synthesized to detect 13 bacterium species and (40) clinical specimens. All the tested bacterium species were positive. The lowest concentration of Escherichia Coli detected by serial dilution was 4 CFU.ml-1. The positive rate of PCR (27/40) was higher than that of bacterium culture(18/40). The results indicate that these PCR primers possess high specificity and sensitivity in identifying 16S rRNA gene of bacteria. |
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