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鼻咽癌中与已知基因同源的差异表达cDNA序列
引用本文:湛凤凰 李桂源. 鼻咽癌中与已知基因同源的差异表达cDNA序列[J]. 湖南医科大学学报, 1999, 24(2): 103-106
作者姓名:湛凤凰 李桂源
摘    要:
采用新近发展的cDNA代表性差异分析法筛选鼻咽癌中不表达的或表达降低的cDNA序列。结果显示:有9个与已知基因高度同源的cDNA序列。通过对这些已知基因的结构和功能分析,发现有与细胞骨架成分相关的基因:a-actinin,ezrin和细胞角蛋白13;

关 键 词:鼻咽肿瘤 抑瘤基因 cDNA-RDA 基因克隆

Differentially expressed cDNA sequences homologous with known genes in human nasopharyngeal carcinoma]
F Zhan,L Cao,C Hu,G Li. Differentially expressed cDNA sequences homologous with known genes in human nasopharyngeal carcinoma][J]. Bulletin of Hunan Medical University, 1999, 24(2): 103-106
Authors:F Zhan  L Cao  C Hu  G Li
Affiliation:Cancer Research Institute, Hunan Medical University, Changsha 410078.
Abstract:
In order to search the tumor suppressor genes correlated with pathogenesis of human nasopharyngeal carcinoma(NPC), we applied the PCR-based subtractive hybridization technique of representational difference analysis (RDA) to the primary culture cells of normal human nasopharyngeal epithelial and HNE1, a poorly differentiated NPC cell line. Following four successive subtractions of HNE1 complementary DNA from normal human nasopharyngeal epithelial cells complementary DNA, difference products were cloned into pGEM-T easy vector and nucleotide sequences determined. Comparison of cDNA sequences against the databases identified 9 known genes. Known genes included TRIP1(TGF beta receptor interacting protein), TAF, ezrin, MHC II, actinin, Histone H1 zero, Cytokeratin 13, Squalene Synthetase and RNA Synthetase-like. Some of them have an effective suppressive ability on the tumor. In this study, we have demonstrated that cDNA RDA is an effective strategy for systematically isolating differences in gene expression between two related but functionally distinct cells. Our results also indicated that the NPC includes interaction of multigenes and this experiment offers a new route for NPC research.
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