高效液相色谱法同时测定三七总皂苷中人参皂苷Rg_1、Re、Rb_1与三七皂苷R_1含量

目的建立高效液相色谱法同时测定三七总皂苷中人参皂苷Rg1、Re、Rb1与三七皂苷R1的方法。方法采用高效液相色谱法 ,固定相为氨基键合相 ,流动相为乙腈 水 ( 81∶1 9,V∶V) ,检测波长2 0 3nm。结果三七总皂苷中人参皂苷Rg1、Re、Rb1和三七皂苷R1与其他成分分离良好 ,保留时间分别约为 5 7min、8 9min、2 5 1min和 9 9min。人参皂苷Rg1在 80～ 2 80mg/L(r =0 9992 )、Re在 2 0～ 1 80mg/L(r=0 9993 )、Rb1在 95～ 2 85mg/L(r=0 9991 )、三七皂苷R1在1 8～ 1 4 6mg/L(r=0 9991 )内线性关系良好 ,人参皂苷Rg1、Re、Rb1和三七皂苷R1的回收率分别为 99 1 %、98 4 %、98 6%和 97 1 % ,RSD分别为 2 1 %、2 0 %、2 2 %、2 8%。结论本法可用于三七的质量控制

Determination of the contents of ginsenoside Rg1,Re,Rb1 and the notoginsenoside R1 in the total notoginsenosides of the Panax notogineseng (Burk.)F.H.Chen by HPLC

Objective To determine the contents of the ginsenoside Rg 1,Re,Rb 1 and notoginsenoside R 1 in the notoginsenoside materials.Methods The Asahipak NH 2P-50 column was used with a mobile phase of acetonitrile-water (81∶19,V:V),the wavelength of the detector was set at 203 nm.Results The peaks of ginsenoside Rg 1, Re,Rb 1 and notoginsenoside R 1 appear at about 5.7 min, 8.9 min, 25.1 min and 9.9 min.The linear ranges of the ginsenoside Rg 1,Re,Rb 1 and notoginsenoside R 1 are 80～ 280 mg/L,20～ 180 mg/L,95～ 285 mg/L and 18～ 146 mg/L respectively.The correlation coefficients are 0.999 5, 0.999 3, 0.999 1 and 0.999 3.The average recovery rates are 99.1%,98.4%,98.6% and 97.1%,the RSD are 2.1%,2.0%,2.2% and 2.8% respectively.Conclusions The method is simple,accurate,with a good reproducibility.And it can be applied to the quality control of Panax notoginseng.