青藤碱对类风湿关节炎患者滑膜细胞周期的影响

目的探讨中药青藤碱(SN)治疗类风湿关节炎(RA)的相关机制,研究SN诱导滑膜细胞凋亡与细胞周期改变的关系。方法姬姆萨染色光学显微镜下观察滑膜细胞凋亡形态的变化,流式细胞仪检测细胞凋亡率、细胞周期的表达。结果光镜下可见细胞变圆、细胞质红染、核固缩等典型凋亡形态学变化。流式细胞仪结果显示,SN诱导滑膜细胞凋亡并主要作用于细胞周期G1—S期。结论SN可能通过诱导细胞凋亡而抑制滑膜细胞增殖,这种作用与细胞周期有关,这些有助于将来治疗类风湿关节炎方案的合理设计。

Effect of sinomenine on cell cycles of synoviocytes in patients with rheumatoid arthritis

Objective It is to explore the correlated treatment mechanism of sinomenine(SN) on rheumatoid arthritis(RA),and to explore the relationship between synoviocytes apoptosis induced by SN and change of cell cycles.Methods The morphological changes of synoviocytes stained by Giemsa staining were observed under light microscope.The rate of synoviocytes apoptosis and the changes of cell cycle were measured by flow cytometry.Results The classic morphological changes of apoptosis,such as cell rounding,cytoplasm red stain and shrinkage could be seen under light microscope.The results of flow cytometry showed that SN could induce apoptosis of synoviocytes and play its action mainly in G1 to S phase of cell cycle.Conclusion SN can induce apoptosis of synoviocytes due to inhibit proliferation of the cells.This function mey be related with cell cycle,and may be helpful for designing an appropriate therapeutic project of RA in future.