| Effect of rhBMP-2 and Osteogenic Revulsants on Proliferation and Differentiation of Bone Marrow Stromal Cells in Rats |
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| 作者姓名： | 方煌 杨小荣 陈安民 罗永湘 |
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| 作者单位： | Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China |
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| 摘 要： | The effects of recombinant human bone morphogenetic protein-2 （rhBMP-2） and osteogenic revulsants alone or in combination at different time points and in different dosages on proliferation and osteogenesis of bone marrow stromal cells （BMSCs） in SD rats were investigated. Rat BMSCs were cultured in vitro and induced by rhBMP-2 in different dosages （10, 50, 100 and 200μg/L） alone or in combination with osteogenic revulsants. MTT colorimetric assay was used to evaluate The proliferation, activity of alkaline phosphoric （ALP） and osteocalcin were measured at 3rd, 6th, 9th, 12th day respectively. The results showed that rhBMP-2 and osteogenic revulsants could promote the differentiation of BMSCs towards osteoblast phenotype. The proliferation of BMSCs could be enhanced by rhBMP-2 in a dose-dependent manner. The expression of osteoblast phenotype was significantly higher by using both of them than by using them alone, which was verified by the activity of ALP and osteocalcin. It was suggested that the combined use of rhBMP-2 and osteogenic revulsants could promote the proliferation and simultaneously induce and maintain the expression of osteoblast phenotype of BMSCs in rats.
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| 关 键 词： | 骨髓干细胞 细胞增殖 骨生成 动物模型 |
| 收稿时间： | 2006-10-11 |
Effect of rhBMP-2 and osteogenic revulsants on proliferation and differentiation of bone marrow stromal cells in rats |
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| Fang?Huang,Yang?Xiaorong,Chen?Anmin,Luo?Yongxiang.Effect of rhBMP-2 and Osteogenic Revulsants on Proliferation and Differentiation of Bone Marrow Stromal Cells in Rats[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(5):561-563. |
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| Authors: | Fang Huang Yang Xiaorong Chen Anmin Luo Yongxiang |
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| Institution: | Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Summary The effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) and osteogenic revulsants alone or in combination
at different time points and in different dosages on proliferation and osteogenesis of bone marrow stromal cells (BMSCs) in
SD rats were investigated. Rat BMSCs were cultured in vitro and induced by rhBMP-2 in different dosages (10, 50, 100 and 200 μg/L) alone or in combination with osteogenic revulsants.
MTT colorimetric assay was used to evaluate The proliferation, activity of alkaline phosphoric (ALP) and osteocalcin were
measured at 3rd, 6th, 9th, 12th day respectively. The results showed that rhBMP-2 and osteogenic revulsants could promote
the differentiation of BMSCs towards osteoblast phenotype. The proliferation of BMSCs could be enhanced by rhBMP-2 in a dose-dependent
manner. The expression of osteoblast phenotype was significantly higher by using both of them than by using them alone, which
was verified by the activity of ALP and osteocalcin. It was suggested that the combined use of rhBMP-2 and osteogenic revulsants
could promote the proliferation and simultaneously induce and maintain the expression of osteoblast phenotype of BMSCs in
rats. |
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| Keywords: | rhBMP-2 BMSCs proliferation osteogenic differentiation |
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