线粒体融合素基因-2对肝癌细胞Caspase蛋白表达的影响及意义 |
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引用本文: | 王尧,陈艳红,陈宏,郭志刚. 线粒体融合素基因-2对肝癌细胞Caspase蛋白表达的影响及意义[J]. 广州医药, 2009, 40(4): 3-5. DOI: 10.3969/j.issn.1000-8535.2009.04.002 |
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作者姓名: | 王尧 陈艳红 陈宏 郭志刚 |
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作者单位: | 广东省中山市人民医院普外三科,528403;广东省中医院,510120 |
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摘 要: | 目的研究外源性线粒体融合素基因-2(mitofusin-2 gene,mfn2)对肝癌细胞Caspase蛋白表达的影响及意义。方法用脂质体2000将质粒pEGFPmfn2、空质粒pEGFP-N2分别转染肝癌细胞株HepG2,分别检测转染后48h HepG2细胞mfn2 mRNA的表达、蛋白表达及凋亡特异性蛋白酶Caspase3、Caspase 9及proCaspase 9蛋白表达的变化。结果经脂质体2000转染后,HepG2细胞基因组中存在有目的基因特异性片段,转染后细胞中有Mfn2蛋白表达;转染pEGFPmfn2组细胞中Caspase 3、Caspase 9表达明显增加(P〈0.05),而proCaspase 9的表达明显减少(P〈0.05)。结论外源性mfn2基因转染可激活Caspase 3、Caspase 9,进而激活细胞凋亡信号通路,这可能是mfn2基因诱导肝癌细胞凋亡的可能机制。
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关 键 词: | 线粒体基因 肝癌细胞 细胞转染 Caspase蛋白 |
The effect and significance of exogenous mitofusin-2 gene on expression of Caspase of hepatocellular carcinoma cells |
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Affiliation: | Wang Yao, Chen Yanhong, Chen Hong, et al.( Department of General Surgery, Zhongshan Municipal People's Hospital, Zhongshan 528403, China ) |
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Abstract: | Objective To investigate the effect and significance of exogenous mitofusin-2 gene (mfn2) on expression of Caspase of hepatocellular carcinoma cells. Methods Plasmid pEGFPmfn2 and pEGFP-N2 were transfected respectively into hepatocellular carcinoma cell strain HepG2 by Lipofectamine^TM 2000 in vitro. The mRNA expression, protein expression and the expression of apoptosis related protein expression of Caspase 3, Caspase 9 and proCaspase 9 were detected. Results After transfected by Lipofectamin^TM 2000, the genome of mfn2-transfected cells contained a 392bp specific fragment of mfn2 gene which indicated the succeessful transfection, and successful protein expression was confirmed by Western Blot assay. The protein expression of Caspase 3, Caspase 9 were higher and procaspase 9 were lower in mfn2 transfected cells than in control group cells (P 〈 0. 05 ) . Conclusion Mfn2 could activate Caspase 3 and Caspase 9 which might contribute to explore the mechanism of its inducing apoptosis of hepatocellular carcinoma ceils. |
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Keywords: | Mitochondrial gene Carcinoma Hepatocellular Cell transfection Caspase |
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