连翘水煎液对大鼠离体肠管运动作用的实验研究

目的:研究连翘水煎液对大鼠离体回肠运动的作用。方法:将大鼠离体回肠作为研究对象,以氯化乙酰胆碱、氯化钡(Ba Cl2)、多巴胺(DA)、肾上腺素(Adr)、硫酸阿托品(Atr)为工具药,利用生物信号采集处理系统,研究连翘水煎液对大鼠离体回肠的作用。结果:连翘水煎液能显著降低正常大鼠离体回肠的自主运动,体现在收缩张力和振幅降低,并呈现明显量效关系。氯化乙酰胆碱和Ba Cl2能使肠肌收缩,升高其张力和振幅,不同浓度连翘均能拮抗氯化乙酰胆碱和Ba Cl2造成的肠肌收缩,使其张力和振幅降低,差异有统计学意义(均P0.05)。DA,Adr,Atr能使肠管运动抑制,体现在张力和振幅变小,不同浓度的连翘水煎液均能使DA、Adr和Atr组的张力进一步降低,振幅进一步变小,差异有统计学意义(均P0.05),表现出对肠管运动的协同抑制作用。结论:连翘对肠管运动具有抑制作用,其机制可能与拮抗M胆碱受体、拮抗钙离子、激动多巴胺受体、激动肾上腺素α和β受体有关,也可能是对肠管的直接抑制作用。

Experimental Research of Fructus Forsythiae Decoction on the Intestinal Motility of Rat In Vitro

Objective:To observe the effect of Fructus forsythiae decoction on rat ileum activity in vitro.Methods:The isolated ileum of rats were used as the research object. Chloride acetyl chloride(Ba Cl2),dopamine(DA),adrenaline(Adr) and atropine sulfate(Atr) were used as agents,and the biological signal acquisition and processing system was applied to study the effect of Fructus forsythiae decoction on rat ileum in vitro. Results:Fructus forsythiae decoction could significantly reduce the isolated rat ileum autonomous motility,manifesting as the reduction of tension and amplitude of contraction,with a significant dose-effect relationship. Acetylcholine chloride and Ba Cl2 could make intestinal muscle contraction,increase its tension and amplitude,while Fructus forsythiae decoction of different concentrations could antagonize the intestinal contraction caused by acetylcholine chloride and Ba Cl2,with the decreased the tension and amplitude(P<0.05).DA,Adr and Atr could inhibit the intestinal motility,manifesting as reduced tension and amplitude,and Fructus forsythiae decoction of different concentrations could further reduce the tension and amplitude in DA,Adr and Atr groups(all P<0.05),indicating the synergistic inhibition of the intestinal tube. Conclusion:Fructus forsythiae decoction has inhibitory effect on intestinal motility,and its mechanism may be related to the antagonism of M cholinergic receptor,antagonism of calcium ion,activation of dopamine receptor,activation of adrenergic α and β receptors,and may also be a direct inhibitory effect on intestinal tube.