骨碎补总黄酮对急性肾衰竭大鼠ICAM-1基因表达的影响

目的：研究骨碎补总黄酮（AFFDR）对大肠杆菌脂多糖（LPS）诱导的急性肾衰竭大鼠肾组织细胞间黏附因子-1（ICAM-1）mRNA表达的影响。方法：用LPS（30mg/kg）腹腔注射建立急性肾衰竭大鼠模型,分模型组（LPS组）、骨碎补总黄酮治疗组（LPS＋AFFDR组）、正常对照组（Control组）和骨碎补对照组（AFFDR组）。实验第7天测定各组大鼠血清肌酐（Scr）的含量,观察肾脏的超微结构和ED-1阳性巨噬细胞的浸润,并用半定量RT-PCR方法检测AFFDR对肾组织ICAM-1 mRNA表达的影响。结果：LPS引起大鼠Scr明显升高,肾脏近曲小管出现明显病理改变。AFFDR有效降低LPS攻击大鼠Scr的含量,明显减轻近曲小管的损伤。LPS组肾组织ED-1阳性巨噬细胞浸润和ICAM-1 mRNA的表达明显高于对照组,而LPS＋AFFDR组肾组织ED-1阳性巨噬细胞的浸润和ICAM-1 mRNA的表达明显低于LPS组。结论：AFFDR防治内毒性急性肾衰竭的作用机制可能与其抑制肾组织ED-1阳性巨噬细胞浸润和ICAM-1的表达有关。

Effect of Assemble flavones of Fortune''s drynaria Rhizome on the Expression of ICAM-1 in Rats with Acute Renal Failure

Objective： To investigate the effect of Assemble Flavones of Fortune＇s Drynaria Rhizome（AFFDR） on the mR- NA expression of renal intercellular adhesion molecule - 1 （ ICAM - 1 ） in rats with acute renal failure induced by lipopolysaecharide （LPS）. Methods：Acute renal failure rats model were established by intraperitoneal injection with LPS（30 mg/kg）,and then divided into LPS group,AFFDR treatment group （LPS ＋ AFFDR group） .normal control group and AFFDR group. On the 7th day after experiment, blood was collected for determining creatinine （Scr） concentrations, renal tissue for examining the expression of ED- 1 positive maerophage. In addition, electron microscopy was used to examine the ultrastructure changes in kidney, and RT- PCR was performed to detect renal mRNA expression of intercellular adhesion molecule - 1 （ICAM - 1 ）. Results： LPS significantly increased Ser concentrations, and produced an obvious pathological change in renal ultrastructure, which was significantly attenuated by AFFDR treatment. Moreover,AFFDR treatment markedly suppressed an increase in renal ICAM- 1 mRNA expression induced by LPS. Conclusion：These results suggest that AFFDR protects against LPS - induced acute renal failure through inhibiting ED- 1 positive macrophage infiltration and ICAM- 1mRNA expression in renal tissue.