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| 肾癌组织中Tim-3基因的甲基化分析 | |
| 引用本文： | 郑伏甫,陈恩静,李晓飞,邓春华,戴宇平,丘少鹏. 肾癌组织中Tim-3基因的甲基化分析[J]. 中国医学文摘(检验与临床), 2011, 0(6): 352-353,355 |
| 作者姓名： | 郑伏甫 陈恩静 李晓飞 邓春华 戴宇平 丘少鹏 |
| 作者单位： | [1]中山大学附属第一医院泌尿外科,广州510080 [2]中山大学附属第一医院内科,广州510080 |
| 基金项目： | 广东省科技计划项目基金（2010B080701059） |
| 摘 要： | 目的分析肾癌组织中Tim-3基因启动子的甲基化情况,探讨Tim-3甲基化在肾癌发生中的可能作用。方法采用甲基化特异性PCR（MSP）检测20例肾癌组织和3例癌旁组织中Tim-3基因启动子甲基化状态,分析检测结果。结果肾癌组织中有11例（55%）检测到了Tim-3基因甲基化表达,相应癌旁组织中没有检测到Tim-3基因甲基化表达。结论肾癌组织中Tim-3基因启动子发生甲基化,Tim-3基因甲基化可能与肾癌的发生发展有关。 |
| 关 键 词： | 肾肿瘤 DNA甲基化 MSP Tim-3 |
Abnormal methylation of Tim-3 gene in renal carcinoma |
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| ZHENG Fu-fu＊,CHEN En-jing,LI Xiao-fei,DENG Chun-hua,DAI Yu-ping,QIU Shao-peng. Abnormal methylation of Tim-3 gene in renal carcinoma[J]. , 2011, 0(6): 352-353,355 | |
| Authors: | ZHENG Fu-fu＊ CHEN En-jing LI Xiao-fei DENG Chun-hua DAI Yu-ping QIU Shao-peng |
| Affiliation: | .＊Department of Urology,the First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China |
| Abstract: | Objective To investigate the methylation level of the promoter of Tim-3 gene in the renal carcinoma tissues and to assess its role in the renal carcinoma genesis.Methods Methylation specific PCR （MSP） method was used to analysis the methylation status of the promoter of Tim-3 gene in the renal carcinoma tissues of 21 patients with renal carcinoma and 3 corresponding adjacent normal tissues.Results Tim-3 gene methylation found in 11 （52.4%）） of the renal carcinoma tissues and no abnormal Tim-3 gene methylation found in the 3 adjacent normal tissues.Conclusions Hypermethylation of Tim-3 gene promoter is present in the renal carcinoma tissues,suggesting that hypermethylation of Tim-3 maybe associated with the tumorigenesis of renal carcinoma. |
| Keywords: | Kidney neoplasms DNA methylation MSP Tim-3 |
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