人类炎性乳腺癌移植肿瘤裸鼠动物模型的建立和生物学特性的研究

目的 为研究肿瘤的转移机制,建立炎性乳腺癌裸鼠动物模型,并检测其生物学特性。方法 应用Northern印迹、Western印迹、酶谱分析法和免疫组化法检测炎性乳腺癌裸鼠动物模型的生物学特性。结果 成功地建立了炎性乳腺癌裸鼠动物模型。并证实该动物模型表现为人类炎性乳腺癌的生物学特征,同时也发现该肿瘤动物模型有肺部的远处转移,对其生物学特性的检测中发现该移植肿瘤表现为雌激素受体阴性、孕激素受体阴性、p53强阳性,表皮生长因子受体过度表达以及Her-2/neu过度表达等。移植肿瘤形成率为70%左右,同时进行了一系列初步的筛选实验,和其他非炎性乳腺癌移植肿瘤相比,炎性乳腺癌移植肿瘤表达非常高的钙粘着糖蛋白和粘蛋白,而其他因子则无异常表达。结论 炎性乳腺癌的裸鼠移植肿瘤动物模型为研究肿瘤转移机制提供了很好的研究模型。

Establishment and characterization of human inflammatory breast carcinoma neoplasm transplantation in nude mice

Objective To establish and char a cterize human inflammatory breast cancer xenograft in nude mice. Methods Animal studies, Northern and Western blot, zymograms and immunohistochemistry were used in our studies. Results Human transplantable inflammatory carcinoma were established in nu de mice. This xenograft as its human counterpart exhibited striking erythema of the overlying skin, lymphovascular invasion and other similar biological char acteristics. It also exhibited lung metastasis 4-6 weeks after growth. This xen ograft was ER, PR, Her-2/neu negative and p53, EGFR positive. Comparative studi es of inflammatory breast cancer xenograft with non-inflammatory xenograft indi cated 10-20 fold overexpression of E-cadherin and MUC1. Concl usions This human inflammatory breast carcinoma xenograft will provide an excellent animal model to allow us to further dissect out both the up stream regulatory machinery and downstream effector molecules responsible for th e inflammatory carcinoma phenotype.