四甲基偶氮唑蓝法体外药敏试验在优化胃癌治疗方案中的应用

 【摘要】　目的　探讨不同化疗药物之间对胃癌细胞的敏感性差异，以筛选出最佳的化疗药物或联合化疗方案。方法　应用四甲基偶氮唑蓝（MTT）显色分析法测定34例胃癌标本对7种化疗药物及其组成的联合化疗方案的敏感性或耐药性，并比较结果。结果　胃癌化疗敏感性个体差异较大，联合化疗药物的敏感性明显优于单药（P＜0.01）。各药物平均抑制率由高到低的顺序依次为5-Fu+MMC+ADM＞5-Fu+CF+VP16+ DDP+MMC＞5-Fu＞5-Fu+CF+VP16＞5-Fu+CF+VP16+DDP＞5-Fu+MMC+EPI＞ADM＞EPI＞MMC＞VP16＞DDP＞CF。无论是单药组间比较，还是联合药物组间比较，其敏感性的差异均无统计学意义（均P＞0.05）。结论　体外肿瘤细胞药敏试验对临床肿瘤化疗用药有很强的预见性和指导性，并能发现耐药病例。对胃癌患者进行化疗时，应尽量选择联合用药方案。

Applied study on optimizing therapeutic regimen of gastric cancer with the chemosensitivity test in vitro by MTT assay

【Abstract】　Objective　To investigate the different sensitivities of commonly used chemotherapeutic drugs on gastric cancer by the methed of primary culture of gastric cancer cells, and to try to screen the best chemotherapeutic drugs or combined drugs. Methods　The drug sensitivity or resistance of 34 gastric cancer samples to 7 chemotherapeutic drugs and compositive combined chemotherapy were detected by MTT colori－metric assay, and compared the results. Results　The individual chemosensitivity was comparatively different. The chemosensitivity of combined drugs was apparently superior to single drugs, P <0.01.The sequence for the average inhibition ratio of drugs was 5-Fu+MMC+ADM>5-Fu+CF+VP16+DDP+MMC>5-Fu>5-Fu+CF+VP16>5-Fu+CF+VP16+DDP>5-Fu+MMC+EPI>ADM>EPI>MMC>VP16>DDP>CF. There were no significant difference in sensitivity among the single drug groups and among the combined drug groups, all P >0.05. Conclusion　The tumour chemosensitivity test in vitro gave some prediction and guidances for the clinic chemotherapy,and it could discover the drug resisting cases. The combined chemotherapy should be selected for gastric carcino－ma patients．