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| 沙利度胺对自身免疫性甲状腺炎大鼠动物模型外周血CXCR3和CCR4表达的影响 | |
| 引用本文： | 侯强,徐晓光,张红,潘叶飞,顾军. 沙利度胺对自身免疫性甲状腺炎大鼠动物模型外周血CXCR3和CCR4表达的影响[J]. 中国皮肤性病学杂志, 2011, 25(7) |
| 作者姓名： | 侯强 徐晓光 张红 潘叶飞 顾军 |
| 作者单位： | 1. 上海徐汇区大华医院皮肤科,上海,200237 2. 第二军医大学长海医院皮肤科,上海,200433 3. 青岛市海慈医疗集团皮肤科,山东青岛,266033 4. 张家港澳洋医院皮肤科,江苏张家港,215600 |
| 摘 要： | 目的分析沙利度胺对实验性自身免疫性甲状腺炎大鼠外周血中CXCR3和CCR4表达的影响,探讨其调节Th1/Th2平衡的可能机制。方法用20只清洁级雌性Wistar大鼠建立实验性自身免疫性甲状腺炎动物模型,并将其随机分成两组,即模型对照组与沙利度胺组;另取10只清洁级雌性Wistar大鼠不作预处理,将其设为正常对照组。正常对照组及模型对照组大鼠每天给予生理盐水灌胃,沙利度胺组大鼠给予沙利度胺片悬液6.875mg/(kg.d)灌胃处理,4周后处死大鼠,采集血清,同时提取外周血单核细胞,以Th1-Th2-Th3Microarray基因芯片、realtime RT-PCR和ELISA法检测其CXCR3和CCR4基因及蛋白水平差异。结果与正常对照组相比,模型对照组大鼠外周血CXCR3和CXCR3/CCR4比值升高,CCR4明显下降;而经沙利度胺处理后,其CXCR3和CCR4表达均下降,CXCR3/CCR4比值也明显降低,差异均有统计学意义(P均<0.01)。结论沙利度胺可同时下调CXCR3和CCR4的表达,以CXCR3为甚,从而诱导实验性自身免疫性甲状腺炎大鼠体内不同Th细胞的迁移,在一定程度上起到辅助调节Th1/Th2平衡的作用。 |
| 关 键 词： | 沙利度胺 甲状腺炎,自身免疫性 Th1 Th2 基因芯片 CXCR3 CCR4 |
Impact of Thalidomide on Expressions of CXCR3 and CCR4 in Peripheral Blood of Model rats with Autoimmune Thyroiditis |
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| HOU Qiang,XU Xiao-guang,ZHANG Hong,PAN Ye-fei,GU Jun. Impact of Thalidomide on Expressions of CXCR3 and CCR4 in Peripheral Blood of Model rats with Autoimmune Thyroiditis[J]. The Chinese Journal of Dermatovenereology, 2011, 25(7) | |
| Authors: | HOU Qiang XU Xiao-guang ZHANG Hong PAN Ye-fei GU Jun |
| Affiliation: | HOU Qiang1,XU Xiao-guang2,ZHANG Hong3,PAN Ye-fei4,GU Jun2(1.Department of Dermatology,Dahua Hospital,Xuhui District,Shanghai 200237,China,2.Department of Dermatology,Changhai Hospital,Second Military Medical University,Shanghai 200433,3.Department of Dermatology,Haici Medical Group,Qingdao 266033,4.Department of Dermatology,Aoyang Hospital,Zhangjiagang 215600,China) |
| Abstract: | Objective To analyze the impact of thalidomide on expression of CXCR3 and CCR4 in peripheral blood of experimental autoimmune thyroiditis(EAT)rats,and to explore its possible mechanism in regulating Th1/Th2 balance.Methods EAT models were established in 20 female Wistar rats,which were then randomly divided into two groups:control model group and thalidomide group.Another 10 rats were set as normal group.Rats of normal and control model groups were given normal saline by gavage per day,while thalidomide gro... |
| Keywords: | Th1 Th2 CXCR3 CCR4 |
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