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| 神经生长因子对大鼠前脑缺血再灌注后细胞凋亡及FaS蛋白表达的影响 | |
| 引用本文： | 高红华,沈雪莉. 神经生长因子对大鼠前脑缺血再灌注后细胞凋亡及FaS蛋白表达的影响[J]. 中国临床神经科学, 2008, 16(6): 595-600 |
| 作者姓名： | 高红华 沈雪莉 |
| 作者单位： | 1. 中国医科大学附属第四医院神经科,110032 2. 中国医科大学附属第一医院神经科,110001 |
| 摘 要： | 目的：研究神经生长因子（NGF）对大鼠前脑缺血再灌注后海马CA1区Fas蛋白表达和细胞凋亡的影响。方法：夹闭大鼠双侧颈总动脉造成前脑缺血，30min后松夹再灌注，NGF组和生理盐水组于再灌注开始时分别肌肉注射NGF30μg·mL^-1和生理盐水0．1mL，应用免疫组化法和TUNEL法检测各组大鼠海马CA1区Fas蛋白表达和细胞凋亡。结果：再灌注后6和24h，NGF组Fas蛋白平均吸光度值小于生理盐水组（P〈0．001和P〈0．05）。再灌注后48h两组比较差异无统计学意义（P〉0．5）。再灌注后6、24和48h，NGF组TUNEL阳性细胞率均低于生理盐水组，差异有统计学意义（P〈0．005）。结论：NGF可以减少大鼠脑缺血再灌注后海马CA1区Fas蛋白表达，抑制细胞凋亡，从而发挥其神经保护作用。 |
| 关 键 词： | 脑缺血再灌注 细胞凋亡 神经生长因子 Fas蛋白 |
Effects of Nerve Growth Factor on the Expression of Fas Protein and Cell Apoptosis in the Hippocampus Following Cerebral Ischemia and Reperfusion in Rats |
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| GAO Hong-Hua,SHEN Xue-Li. Effects of Nerve Growth Factor on the Expression of Fas Protein and Cell Apoptosis in the Hippocampus Following Cerebral Ischemia and Reperfusion in Rats[J]. Chinese Journal of Clinical Neurosciences, 2008, 16(6): 595-600 | |
| Authors: | GAO Hong-Hua SHEN Xue-Li |
| Affiliation: | GAO Hong-Hua~1 SHEN Xue-Li~2 ~1Department of Neurology,the Fourth Clinical Hospital,China Medical University,Shenyang 110032,~2Department of Neurology,the First Clinical Hospital,Shenyang 110001,China |
| Abstract: | Aim:To study the effects of nerve growth factor on the expression of Fas protein and cell apoptosis in the hippocampus following cerebral ischemia and reperfusion in rats.Methods:The model of ischemic/reperfusion is established by clipping bilateral cervical arteries for 30 minutes and then removing the clips.The rats in nerve growth factor(NGF)and normal saline(NS)groups were given intramuscular injection of NGF and NS respectively at the beginning of reperfusion.Immunohistochemistry staining is used to de... |
| Keywords: | cerebral ischemia reperfusion apoptosis nerve growth factor Fas protein |
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