溶血磷脂酰胆碱对牛视网膜微血管内皮细胞NO和NOS生成的影响

目的:探讨溶血磷脂酰胆碱(lysophosphatidylcholine,LPC)对牛视网膜微血管内皮细胞(bovine retinal microvascular endothelial cells,BRECs)一氧化氮(nitric oxide,NO)和一氧化氮合酶(nitric oxide synthase,NOS)生成的影响.方法:原代培养的牛视网膜微血管内皮细胞分为4组:对照组,LPC(20 μmol/L、40 μmol/L、60μmol/L)3组;LPC各组根据不同作用时间(6 h、12 h、24 h)又分为3个亚组,用NO和NOS试剂盒检测各组内皮细胞培养上清液中NO含量和NOS活性.结果:LPC可使BRECs条件培养基中NO含量逐渐减少,NOS活性逐渐减弱,具有时间和剂量依赖性,LPC(60μmol/L)作用24 h时效果最明显,差异有统计学意义(P＜0.05).结论:LPC可能通过抑制BRECs生成NO和减弱NOS活性而促进糖尿病视网膜病变的发生发展.

Effect of Lysophosphatidyl Choline on Production of Nitric Oxide and Nitric Oxide Synthase in Bovine Retinal Microvascular Endothelial Cells

Objective: To study the effects of lysophosphatidylcholine(LPC) on the production of nitric oxide(NO) and nitric oxide synthase(NOS) in cultured bovine retinal microvascular endothelial cells(BRECs).Methods: The primary BRECs were divided into 4 groups: the control group and 3 LPC groups according to the doses used(20 umol/L,40 umol/L,and 60 umol/L).Each LPC group was divided again into 3 subgroups depending on incubation time.NO production and NOS activity in conditioned media in which BRECs were cultured were assessed with NO and NOS kits.Results: In LPC groups,NO production and inhibited NOS activity decreased significantly(P<0.05).The changes showed time and dosage dependence.Conclusion: LPC may play an important role in the development of diabetic retinopathy through inhibiting activity of NOS and decreasing NO production.