rhBMP-2微球温敏型凝胶剂的药剂学特征及对人PDLCs增殖的影响

目的：观察rhBMP-2微球温敏型凝胶的药剂学特征及对人牙周膜细胞（PDLCs）增殖的影响。方法：采用W／O／W型乳化溶剂挥发法以聚乳酸-乙醇酸共聚物（PLGA）为材料制备空白微球,通过吸附法制备rhBMP-2载药微球。以单甲基聚乙二醇-羟基乙酸共聚物（MPEG—PLGA）为材料制备凝胶,采用夹心酶联免疫吸附法测定释药速度。采用四唑盐比色法（MTT）及酶动力学法测定细胞增殖和碱性磷酸酶活性。结果：rhBMP-2微球呈规则球形,表面多孔,粒径为（19．6±2.3）μm,微球凝胶剂相转变温度为36℃左右,微球凝胶剂体外释药曲线符合Higachi方程,28d的累积释放度为80％。rhBMP-2微球凝胶剂能明显促进牙周膜细胞的增殖和提高ALP活性,随时间推移在第10d时rhBMP微球凝胶促PDLCs生长的作用明显强于rhBMP溶液。结论：rhBMP-2微球温敏型凝胶剂具有明显的缓释特征,与溶液剂相比促进PDLCs增殖作用更持久。

Characteristics of recombinant human bone morphogenetic protein-2 Loaded microspheres/thermosensitive hydrogels complex and its effects on proliferation of human periodontal ligament cells

Objective:To study the characteristics of rhBMP-2 loaded microspheres/thermosensitive hydrogels com-plex and it effects on proliferation of human periodontal ligament cells.Method:Blank microspheres were prepared with W/O/W emulsion solvent evaporation method.RhBMP-2 loaded microspheres was prepared with absorption method.Thermosensitive hydrogels were prepared with MPEG-PLGA.Enzyme-linked immunosorbent assay was used to determine drug content and in vitro release rate.MTT method and enzyme dynamics method ...