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肝癌肝移植术前和术中癌细胞血行转移与肿瘤分化、微血管侵犯相关性研究
引用本文:吴迪,郑虹,高东宸,沈中阳. 肝癌肝移植术前和术中癌细胞血行转移与肿瘤分化、微血管侵犯相关性研究[J]. 中国临床医学, 2008, 15(1): 63-65
作者姓名:吴迪  郑虹  高东宸  沈中阳
作者单位:天津市第一中心医院移植外科,东方器官移植中心,天津,300381
基金项目:天津市卫生局重点课题(No.03KY01)
摘    要:
目的:探讨检测血播散肝癌细胞内AFP-mRNA评估肝癌肝移植术前和术中微转移与肿瘤分化、微血管侵犯的相关性。方法:采用RT-PCR联合荧光标记探针杂交法定量检测30例血清AFP阳性肝癌肝移植患者术前、术中血播散肝癌细胞内AFP-mRNA,病理分析肿瘤分化程度及微血管侵犯。结果:30例肝癌肝移植术前与术中肺动脉、外周动脉、外周静脉、门静脉血中播散肝癌细胞内AFP-mRNA阳性率以及拷贝数有显著差异(P<0.05);术前、中肿瘤播散组与无肿瘤播散组的微血管侵犯有显著差异(P<0.01);术前肿瘤播散组与术前无肿瘤播散组肿瘤分化无显著差异(P>0.05);术中肿瘤播散组与术中肿瘤无播散组肿瘤分化有显著差异(P<0.01)。结论:RT-PCR联合荧光标记探针杂交法能够检测到肝癌患者血液中播散肿瘤细胞,并且以肺动脉血阳性率最高;肝移植手术过程可以引起肿瘤细胞播散,且与肿瘤分化、微血管侵犯相关。

关 键 词:肝癌  肝移植  肿瘤播散细胞

Study on the Relationship between the Tumor Tell Disseminating and Tumor Differentiation or Vascular Invasion for Liver Transplantation of HCC Preoperation and during Operation
WU Di,ZHENG Hong,GAO Dongchen,SHEN Zhongyang. Study on the Relationship between the Tumor Tell Disseminating and Tumor Differentiation or Vascular Invasion for Liver Transplantation of HCC Preoperation and during Operation[J]. Chinese Journal Of Clinical Medicine, 2008, 15(1): 63-65
Authors:WU Di  ZHENG Hong  GAO Dongchen  SHEN Zhongyang
Abstract:
Objective:To study the relationship in patients with hepatocellular carcinoma(HCC)for liver transplantation(LT)between disseminated tumor cell and tumor differentiation or vascular invasion.Methods:The disseminated tumor cell was quantitated in 30 patients for LT of HCC by AFP-mRNA fluorescence quantitative PCR combined with RT-PCR technique.Tumor cell differentiation and vascular invasion was diagnosed by pathologist.Results:The most sensitive detection of DTC was through pulmonary artery blood.The positive expression ratio and copy of DTC between pre-operation and during operation was significant(P<0.05);Between tumor cell disseminating and without tumor cell disseminating pre-operation and during operation,it had significant difference of tumor cell microvascular invasion(P<0.01);Between tumor cell disseminating and without tumor cell disseminating during operation,it had significant difference of tumor cell differentiation(P<0.01).Conclusion:The detection of human AFP mRNA is very important in clinical prediction of HCC metastasis.It is useful for assess the tumor cell disseminating,and predict the prognosis of hepatocellular carcinoma.It also has the relationship with differentiation,microvascular invasion.
Keywords:Liver transplantation  Hepatocellular carcinoma  Disseminating tumor cell
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