内源性雌、孕激素与人乳头瘤病毒在宫颈癌发生中的协同作用研究

目的探讨雌、孕激素对宫颈癌发生的作用及其与人乳头瘤病毒(HPV)的协同作用。方法采用热启动PCR和ELISA法，对141例宫颈癌和129名健康妇女进行了HPVs、HPV16、雌二醇(E2)和孕酮(P)含量的测定。结果宫颈癌组HPVs和HPV16的阳性率分别为75．2％和46．8％，显著高于健康对照组；血清中E2和P的平均浓度均为病例组高于对照组，差异有统计学意义，特别是E2水平与宫颈癌之间呈剂量反应关系；E2与HPVs之间呈正相加交互作用，且有统计学意义。结论HPV感染，特别是HPV16感染在宫颈癌发生中起重要作用；内源性雌激素的升高可增加宫颈癌发生的危险性；高水平的雌二醇和HPV感染对宫颈癌的发生可能具有协同作用。

Analysis on synergistic action between estrogen,progesterone and human papillomaviruses in cervical cancer

Objective To explore the effects of estrogen (E_2) and progesterone (P) on cervical cancer and the synergistic action between estrogen,progesterone and human papillomaviruses (HPV). Methods Hoted-start polymerase chain reaction (HS-PCR) was used to detect HPVs, HPV16 and ELISA was used to assay E_2 and P on 141 cases with cervical cancer and on 129 healthy controls. Results Positive rates of HPVs and HPV16 were 75.2 % and 46.8 % respectively in cervical cancer group, significantly higher than that in controls. Levels of estrogen and progesterone in case group were significantly higher than that in controls and a dose-responded relationship between the levels of estrogen and cervical cancer was revealed. Estrogen and HPV showed an additive interaction in the development of cervical cancer. Conclusion HPV16 infection played a principal role in the development of cervical cancer. The high levels of entogenous estrogen could increase the risk of cervical cancer and might serve as a cofactor in the development of HPV-induced cervical cancer.