活犀角与犀角抗惊厥作用的研究

目的：通过研究活犀角与犀角对小鼠的抗惊厥作用，探讨活犀角是否可以作为犀角的代用品。方法随机将280只小鼠（自主活动实验160只，抗惊厥实验120只），分为活犀角与犀角各4个剂量组（700、350、175、90 mg· kg－1），另设1个空白对照组和1个地西泮片阳性对照组，分别灌胃给予对应剂量的试验样品，连续2 d。末次给药后30 min后用自主活动记录仪记录各组20只小鼠自主活动；同时对各组另外15只小鼠采用尼可刹米诱发动物惊厥，记录小鼠出现惊厥的时间和死亡时间。结果自主活动记录结果显示，4个剂量的活犀角和犀角对小鼠均有兴奋作用；抗惊厥结果显示活犀角700、350、175、90 mg· kg－1和犀角350、175、90 mg· kg－1能明显降低小鼠的惊厥发生率和／或死亡率，且在350、175、90 mg· kg－1剂量下活犀角和犀角对小鼠的抗惊厥作用无显著性差异。结论在该验条件下，在抗惊厥作用方面可初步判断活犀角在350、175、90 mg· kg－1剂量可以作为犀角的代用品进行使用。

A Study of the Anticonvulsive Effects Between Living Rhino Horn and Rhino Horn

Objective To study of the anticonvulsive effects between living Rhino Horn and Rhino Horn and to explore whether living Rhino Horn could be used as a substitute of Rhino Horn.Methods The 280 mice were randomly divided into four groups of living Rhino Horn (700、350、175、90 mg· kg-1), four groups of Rhino Horn (700、350、175、90 mg· kg-1),a blank control group and a positive group of diazepam.The mice were gavaged with the corresponding dose of test sample for 2 consecutive days.Thirty minutes after last gavaged,the autonomic activeties of 20 mice in every group were records by the independent event recorder.The other 15 mice in every group were induced convulsion by Nikethamide.And then, it was recorded that the time of convulsion and the time of death of the mice.Results Independent activities and anticonvulsants, according to the results of Rhino Horn, living Rhino Horn could be have excitation on the mice of blank control group.Live Rhino Horn (700、350、175、90 mg· kg-1)and Rhino Horn (350、175、90 mg · kg-1 ) could significantly reduce the incidence of eclampsia and/or mortality of the mice, and there was no significant difference be-tween live Rhino Horn and Rhino Horn with the same dose (350、175、90 mg· kg-1 ) .Conclusion Under the condition of this exper-iment,It was estimated preliminarily that the anticonvulsive effect of living Rhino Horn at a dosage of 350、175、90 mg· kg-1 was equal to the anticonvulsive effect of the traditional Rhino Horn, and then the living Rhino Horn could be used as a substitute of Rhino Horn.