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| 哌嗪雌酚酮对新生大鼠头盖骨成骨细胞的影响 | |
| 引用本文： | 武晓蓉,李青南,郑虎,翁玲玲.哌嗪雌酚酮对新生大鼠头盖骨成骨细胞的影响[J].中国骨质疏松杂志,2004,10(2):226-229. |
| 作者姓名： | 武晓蓉 李青南 郑虎 翁玲玲 |
| 作者单位： | 1. 510224,广州,广东药学院药学系 2. 广东医学院骨生物研究室 3. 四川大学华西药学院 |
| 摘 要： | 目的 了解新合成化合物——哌嗪雌酚酮对体外培养成骨细胞的影响。方法 应用MTT法、对硝基苯磷酸盐法、von Kossa染色法、流式细胞术及RT-PCR观察哌嗪雌酚酮对体外培养成骨细胞的增殖、分化、矿化结节形成、细胞周期及I型胶原蛋白表达的影响。结果 哌嗪雌酚酮可刺激成骨细胞增殖,刺激骨结节形成(10-7mol/L组,P<0.05)。结论 哌嗪雌酚酮具有刺激体外培养成骨细胞增殖和晚期分化成熟的功能。 |
| 关 键 词： | 哌嗪雌酚酮 新生大鼠 头盖骨 成骨细胞 雌激素替代疗法 骨质疏松症 |
| 修稿时间： | 2003年11月11 |
Effects of piperazinyl estrone on rat calvarial osteoblasts |
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| WU Xiaorong,LI Qingnan,ZHENG Hu,et al..Effects of piperazinyl estrone on rat calvarial osteoblasts[J].Chinese Journal of Osteoporosis,2004,10(2):226-229. | |
| Authors: | WU Xiaorong LI Qingnan ZHENG Hu |
| Institution: | WU Xiaorong,LI Qingnan,ZHENG Hu,et al. Department of Pharmacy,Pharmacal Cottage of Guangdong,Guangzhou 510224,China |
| Abstract: | Objective To study the effects of piperazinyl estrone on osteoblasts cultured in vitro. Meth-ods MTT assay, PNPP assay, von Kossa's staining, flow cytometry and RT-PCR were used to observe the prolif-eration, activity of ALP, the number of mineral nodules , cell cycle and the mRNA expression of I type pro-colla-gen of osteoblasts cultured in vitro. Results Piperazinyl estrone remarkably stimulated the proliferation and in-creared the number of mineral nodules of cultured osteoblasts in 10-7mol/L concentration. Conclusion Piperazinyl estrone stimulates the prolifreation, late differentiation and maturation of osteoblasts cultured in vitro. |
| Keywords: | Piperazinyl estrone Cell culture Osteoblast |
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