Kupffer细胞对日本血吸虫病肉芽肿期CD4~+CD25~+T细胞的影响

目的探讨Kupffer细胞对日本血吸虫病肉芽肿期CD4+CD25+T细胞的影响。方法6～8周龄雌性C57BL/6J小鼠30只分为对照组、感染组与感染/氯化钆组3组,每组10只。感染组和感染/氯化钆组小鼠通过腹部感染尾蚴(10条/只),感染/氯化钆组于感染后第4周经尾静脉注射氯化钆,剂量为每次15mg/kg,每周2次;对照组通过尾静脉注射PBS。感染8周后流式细胞仪检测小鼠CD4+CD25+T细胞数量;免疫组织化学染色检测Foxp3的分布;ELISA检测血清细胞因子IL-4、IL-5、IL-10、TGF-β1与IFN-γ的水平,并进行肝功能检测。结果感染组小鼠CD4+CD25+T细胞数量为13.8%,感染/氯化钆组为9.3%;感染组IL-10为41.4pg/ml,感染/氯化钆组为22.6pg/ml;氯化钆可下调Foxp3的分布、血清丙氨酸氨基转移酶的水平,并减轻血吸虫肉芽肿周围的炎症反应。结论Kupffer细胞通过调控CD4+CD25+T细胞数量而参与日本血吸虫肉芽肿的形成。

Effect of Kupffer cells on CD4+CD25+ T cells in schistosome granuloma

[Abstract] ObjectiveTo explore the effect of targeting Kupffer cells on CD4+CD25+ T cells in schistosomiasis granuloma. Methods Six to eight-week-old C57BL/6 female mice were divided into three groups, a control group, an infection group with S. japonicum cercariae and an infection group injected with GdCl3 through the penile vein (15 mg/kg) twice per week. After 8 weeks of the infection, the number of CD4+CD25+Tcells were detected by using flow cytometry and the number of Foxp3 was detected by using immunohistochemistry.For the detection of murine IL-4, IL-5, IL-10, TGF-β1 and IFN-γ, a DuoSet ELISA development kit was used.Results The number of CD4+CD25+Tcells and the level of IL-10 decreased in the infection group injected with GdCl3 comparing with the infection group. GdCl3treatmentdecreased Foxp3 production and the level of ALT, and reduced the inflammatory response in schistosomiasis granuloma. Conclusion Kupffer cells can regulate the response of CD4+CD25+ T cells in schistosomiasis granuloma.