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| 鲍氏针层孔菌原生质体融合育种的研究 | |
| 引用本文： | 曾念开,王秋颖,苏明声. 鲍氏针层孔菌原生质体融合育种的研究[J]. 中药材, 2008, 31(4): 475-478 |
| 作者姓名： | 曾念开 王秋颖 苏明声 |
| 作者单位： | 1. 中国协和医科大学/中国医学科学院药用植物研究所,北京100094;海南医学院药学系,海南海口571101 2. 中国协和医科大学/中国医学科学院药用植物研究所,北京,100094 |
| 基金项目： | 国家科技部社会性公益项目 |
| 摘 要： | 目的：鲍氏针层孔菌优良菌株的选育。方法：通过5.8SrDNA-ITS序列,生长速度、液体发酵生产菌丝体和胞外多糖产量等方面,对不同来源的菌株进行分析比较,筛选出优良的亲本菌株。在此基础上,采用双灭活标记,原生质体PEG诱导融合的方法。结果：筛选出亲本菌株SA02和SA04,以及编号为R002、R003、R005等三个融合菌株与两亲本菌株都具有拮抗反应,且菌落性状表现出不同于亲本的性状。结论：R002、R003和R005初步确定为双亲株的融合子。 |
| 关 键 词： | 鲍氏针层孔菌 桑黄 原生质体融合 育种 针层孔菌 原生质体 融合育种 研究 Protoplast Fusion 融合子 双亲 亲本菌株 性状表现 菌落 拮抗反应 结果 灭活 筛选 比较 分析 来源 糖产量 胞外 菌丝体 |
| 文章编号： | 1001-4454(2008)04-0475-04 |
| 修稿时间： | 2007-09-03 |
The Breeding of Phellinus baumii by Protoplast Fusion |
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| ZENG Nian-kai,WANG Qiu-ying,SU Ming-sheng. The Breeding of Phellinus baumii by Protoplast Fusion[J]. Journal of Chinese medicinal materials, 2008, 31(4): 475-478 | |
| Authors: | ZENG Nian-kai WANG Qiu-ying SU Ming-sheng |
| Affiliation: | Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China. niankaiz@163.com |
| Abstract: | OBJECTIVE: To breed good strains by protoplast fusion in Phellinus baumii. METHODS: 5.8S rDNA-ITS sequences, mycelial growth rate, mycelial biomass and exopolysaccharides (EPS) production of 7 strains in different origins were analyzed, respectively. Then the inactivated parent protoplast and the PEG induction were used in the process of protoplast fusion. RESULTS: The strain P. baumii SA02 and P. baumii SA04 were selected as the parent strains. R002, R003 and R005 had antagonistic effects with parent strains. CONCLUSION: R002, R003 and R005 are fusants of the parent strains. |
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