Interferon-γ Production by Human T Cells and Natural Killer Cells In Vitro in Response to Antigens from the Two Intracellular Pathogens Mycobacterium tuberculosis and Leishmania major |
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Authors: | K. KEMP,L. HVIID,A. KHARAZMI,& M. KEMP |
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Affiliation: | Centre for Medical Parasitology at RHIMA Centre, Copenhagen University Hospital, Rigshospitalet and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark |
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Abstract: | Acquired resistance to both mycobacteria and Leishmania is primarily mediated by interferon-γ (IFN-γ), which triggers mechanisms leading to the death of the microorganism in macrophages. In this study, cell activation and IFN-γ production was investigated in human peripheral blood mononuclear cells (PBMC) from individuals previously sensitized to tuberculin and without known exposure to Leishmania parasites. Immune staining for intracellular IFN-γ and surface markers allowed flow cytometric identification of the cellular sources of IFN-γ in cell cultures incubated with purified protein derivative of tuberculin (PPD) and Leishmania antigens. It was found that IFN-γ was produced in response to both PPD and Leishmania stimulant by T cells in the cultures. Activation of IFN-γ producing natural killer (NK) cells was demonstrated only in some cultures, and only with concomitant T cell activation. |
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