响应曲面法优化南板蓝根中总生物碱的提取工艺

目的:优化南板蓝根中总生物碱的提取工艺.方法:在单因素试验基础上,选取提取时间、乙醇体积分数和液料比3个因素进行Box-Benhnken中心组合设计,以总生物碱提取率为因变量,利用响应曲面法对其提取工艺参数进行优化.结果:优选的提取工艺为提取时间20.23 min,乙醇体积分数83.21％,液料比21.16∶1,南板蓝根总生物碱的最高理论提取率达0.781％.根据生产实际情况,将其修正为提取时间20.5 min,乙醇体积分数83.0％,液料比21∶1,总生物碱提取率(0.78±0.024)％,与理论值较为接近.结论:优化的提取工艺准确可靠,具有一定的实用价值.

Optimization of Extraction Technology for Total Alkaloids from Baphicacanthus cusia by Response Surface Methodology

Objective: To optimize extraction technology of total alkaloids from Baphicacanthus cusia. Method: Based on single factor test,effects of extraction time,ethanol concentration and liquid-solid ratio on yield of total alkaloids from B. cusia was investigated with a three-factor Box-Behnken center-united design,extraction parameters were optimized by response surface methodology. Result: Optimized extraction technology conditions was as following:extraction time 20.23 min,the concentration of ethanol 83.21 %,liquid-solid ratio 21.16: 1,theoretical yield of total alkaloids could be up to 0.781%.According to actual situation in production,optimum extraction parameters were modified as extraction time of 20.5 min,the concentration of ethanol 83.0%,liquid-solid ratio 21.0: 1,validated experiment showed yield of total alkaloids was (0.78 ± 0.024)% under these conditions,which was in consistency with the theoretic value. Conclusion: This modified extraction parameters were credible and reliable with some practical value.