特发性血小板减少性紫癜患儿血清微小病毒B_(19)检测及意义

目的探讨人微小病毒B19(HPVB19)感染与儿童特发性血小板减少性紫癜(ITP)发病的关系。方法采用酶联免疫法(ELISA)对46例ITP患儿利30例健康儿童的血清标本进行HPVB19-IgM、IgG及血小板相关抗体检测。结果46例ITP患儿血清中HPVB19抗体总刚性率43.48%(20/46),30例健康儿童HPVB19-IgM、IgG均为阴性,2组间差异有统计学意义(P<0.01);ITP组中急性型与慢性型之间HPVB19抗体总阳性率差异有统计学意义(P<0.05);病毒感染刚性患儿的血小板相关抗体明显高于病毒感染阴性患儿,差异有统计学意义(P<0.01)。结论ITP患儿血清中HPVB19抗体总刚性率高,尤其是急性型;HPVB19感染后可导致血小板相关抗体升高而致血小板减少。

Detection of serum human parvovirus B19 in children with ITP

Objective To investigate the relationship between parvovirus B19(HPVB19)infection and idiopathic thrombocytopenic purpura(ITP).Methods HPVB19-IgM,IgG and Platelet autoantibodies were measured by enzyme-linked immunosorbent assay(ELISA)in serum specimens from 46 children with ITP and 30 healthy children.Results Twenty of the 46 cases with ITP were positive for HPVB19 antibodies,30 health children were negative for HPVB19 antibodies,there was a significant difference between the groups(P<0.01).The HPVB19 positive rate of the acute stage was higher than that of the chronic stage in the group of ITP(P<0.05).Platelet autoantibodies titer of patients in virus infection were significantly higher than that of patients without viral infection(P<0.01).Conclusion ITP might be associated with HPVB19 infection,especially in the acute stage.Viral infection could lead to platelet autoantibodies rising and platelet count dropping.