β-细辛醚对慢性点燃癫痫大鼠脑组织c-fos表达的影响

目的:研究β-细辛醚对慢性点燃癫痫大鼠脑组织即早基因c-fos表达的影响。方法:大鼠随机分为β-细辛醚高、中、低(200,100,50mg·kg^-1.d^-1)剂量组,阳性药对照组(苯妥英钠36mg·kg^-1),模型对照组(基质),灌胃给药。以青霉素点燃大鼠慢性癫痫,采用westernblot法测定海马c-fos的表达,免疫组化法测定脑皮质c-fos的表达。结果:β-细辛醚能显著增强癫痫大鼠脑内c-fos的表达,且呈量效关系。海马c-fos的表达量:β-细辛醚高、中、低剂量组为(1139.45±155.56),(1109.56±134.03),(1103.73±235.82)CNT.mm²,基质对照组(920.54±203.20)CNT.mm^-2,苯妥英钠组(1106.26±186.24)CNT.mm²。皮质c-fos阳性细胞数:β-细辛醚高、中、低剂量组为(87.1±2.2),(76.3±1.3),(59.9±1.3)个,基质对照组(39.3±2.6)个,苯妥英钠组(95.2±1.1)个。结论:β-细辛醚能增强癫痫大鼠脑内c-fos的表达,是机体重要的抗癫痫反应。

Effects of β-asarone on expression of c-fos in kindling epilepsy rat brain

OBJECTIVE: To study the effects of beta-asarone on expression of immediately early gene c-fos in kindling epilepsy rat brain. METHOD: The rats were randomly divided in to beta-asarone groups (200, 100, 50 mg x kg(-1) x d(-1)), difetoin control group (36 mg x kg(-1)) and model group. The remedy was administered orally. The effects were observed in kindling epilepsy model induced by penicillin, then the expression of c-fos were determined by western blot (hippocampus) and immunohistochemical techniques (cortex). RESULT: Beta-asarone could significantly increase the expression of c-fos in kindling epilepsy rat brain, and show its quantity-effect relation. The expression of c-fos in hippocampus was (1139.45 +/- 155.56), (1109.56 +/- 134.03), (1103.73 +/- 235.82) CNT x mm2 in beta-asarone groups, 920.54 +/- 203.20 in model control group, and 1106.26 +/- 186.24 in difetoin group, respectively. The number of c-fos positive cell was 87.1 +/- 2.2, 76.3 +/- 1.3 and 59.9 +/- 1.3 in beta-asarone groups, 39.3 +/- 2.6 in model control group, and 95.2 +/- 1.1 in difetoin group, respectively. CONCLUSION: Beta-asarone can obviously increase the expression of c-fos in epilepsy rat brain. It is one of important response to epilepsy.