海藻玉壶汤中海藻-甘草反药组合加减应用的急性毒性实验研究

目的: 比较海藻玉壶汤(生甘草)组、海藻玉壶汤(炙甘草)组、去海藻(生甘草)组、去海藻(炙甘草)组、去甘草组、去海藻甘草组的小鼠急性毒性,评价海藻-甘草反药组合在复方中应用的毒性。 方法: 灌胃给与不同配伍组合不同剂量的海藻玉壶汤,计算出LD₅₀,不符合LD₅₀评价条件的组别,采用连续给药28 d,即累计半数致死量的方法来进行毒性评价;眼球采血,通过检测小鼠血清肝、肾功能相关指标评价其对小鼠肝、肾系统的影响。 结果: 各给药组均未得出LD₅₀,海藻玉壶汤(生甘草)组LD₅₀₍₁₆₎为71.08 g·kg^-1,药物剂量21 g·kg^-1;海藻玉壶汤(炙甘草)组LD₅₀₍₃₎为68.28 g·kg^-1,剂量21 g·kg^-1;去甘草组LD₅₀₍₁₂₎为55.78 g·kg^-1,剂量20 g·kg^-1;去海藻(生甘草)组LD₅₀₍₂₂₎为72.27 g·kg^-1,剂量21 g·kg^-1;去海藻甘草组LD₅₀₍₂₁₎为79.62 g·kg^-1,剂量18.1 g·kg^-1;去海藻(炙甘草)组小鼠连续给药28 d仍未达到半数致死量,最大耐受量为生药80 g·kg^-1,药物剂量20 g·kg^-1;给药各组对小鼠的肝、肾脏功能均未见明显影响。 结论: 给药各组LD_50(n)均属无毒的范畴,且对小鼠的肝、肾脏功能指标未见明显影响。

Study of Acute Toxicity Test of Imcompatible Pair of Sargassum and Radix Glycyrrhizae in Haizao Yuhu Decoction

Objective: To evaluate the toxicity of imcompatible pair of Sargassum and Radix Glycyrrhizae in mice by comparing the acute toxicity of different Haizao Yuhu decoction groups. Method: The mice was given different combination and compatibility of Haizao Yuhu decoction with different doses by intragastric administration, and calculated the LD₅₀. If the group didn't meet the LD₅₀ evaluation criteria we would give the drug by continuous intragastic administration for 28 days, ie, we would evaluate the toxity by caculating cumulative median lethal dose. The blood of mice was collected by picking bulb of eye. Detected relevant indicators of liver and kidney function in mice serum to evaluate their influence on liver and kidney. Result: The LD_50(n) of the Haizao Yuhu decoction(Radix Glycyrrhizae) is 71.08 g·kg^-1,the LD_50(n) of the Haizao Yuhu decoction (the prepared Radix Glycyrrhizae) is 68.28 g·kg^-1,the LD_50(n) of the Haizao Yuhu decoction removed Radix Glycyrrhizae is 55.78 g·kg^-1,the LD_50(n) of the Haizao Yuhu decoction removed Sargassum(Radix Glycyrrhizae) is 72.27 g·kg^-1,the LD_50(n) of the Haizao Yuhu decoction removed Sargassum and Radix Glycyrrhizae is 79.62 g·kg^-1. The Haizao Yuhu decoction removed Sargassum (the prepared Radix Glycyrrhizae) still didn't reach median lethal dose after continuous administration for 28 days, the maximum tolerated dose of this group is 80 g·kg^-1;each experimental groups were not showed a significant impact on the liver and kidney functions in mice. Conclusion: The LD_50(n) of each experimental group belongs to non-toxic category, and each group has no significant impact on the liver and kidney system in mice.