益气养阴方及其拆方对急性髓细胞白血病小鼠细胞凋亡及相关蛋白的影响

目的观察益气养阴方及其拆方对小鼠细胞凋亡率及相关蛋白c-myc、survivin的影响,探讨其作用机制。方法 NOD-SCID小鼠经60Co照射后,尾静脉注射KG1a细胞1×106个造模;随机分为对照组、全方组、扶正组、祛邪组。治疗组给予相应药物灌胃,对照组给予等体积生理盐水灌胃。采用流式细胞术检测细胞凋亡率,免疫组化法检测c-myc、survivin蛋白表达。结果与对照组比较,治疗组细胞凋亡率升高,差异有统计学意义(P0.01);治疗组淋巴结c-myc、survivin蛋白表达降低,差异有统计学意义(P0.05)。全方组对各指标的影响均优于扶正组和祛邪组(P0.05)。结论益气养阴方通过降低c-myc、survivin蛋白表达,诱导白血病细胞凋亡,起到治疗作用。

Effect of Yiqi Yangyin Recipe and Its Different Assembling on Apoptosis Rate and Expression of Associated Protein in Acute Myeloid Leukemia Mice

Objective To observe the effects of Yiqi Yangyin Recipe （supplementing qi and nourishing yin） and its different assembling on apoptosis rate and associated protein expression of c-myc and survivin in mice, and explore its mechanism. Methods NOD-SCID mice were injected with 1×10^6 KG 1 a cells from the tail vein after irradiation of 60Co. Model mice were randomly divided into control group, whole recipe group, strengthening body resistance group, eliminating pathogens group. The treatment groups were given correspongding administration, and control group was given equal volume of NS. Flow cytometry was applied to measure apoptosis rate. The immunochemistry method was used to detect the expression of c-myc, survivin. Results Apoptosis rate in all treatment groups were increased （P〈0.01）. The expression of c-myc and survivin in lymph node tissues in all treatment groups were significantly declined compared with control group （P〈0.05）. The effect of whole recipe group on the all indices was superior to strengthening body resistance group and eliminating pathogens group （P〈0.05）. Conclusion Yiqi Yangyin Recipe can decrease the expressions of c-myc and survivin, induce apoptosis in leukemia cells, therefore shows the therapeutic effect.