高海拔地区FISH法检测乳腺癌HER2基因的方法改进

目的 在高海拔地区改良并简化乳腺癌HER2 FISH法检测的预处理步骤,节省实验时间,扩大FISH检测试剂盒的临床应用.方法 以10例HER2免疫组化染色结果为＋ ＋以上的乳腺癌标本为研究对象,采用美国PanPath公司的HER2 FISH检测试剂盒,将其中的预处理步骤改良为高压煮沸处理,比较常规法与改良法对HER2基因扩增结果的影响.结果 两种方法检测乳腺癌HER2基因的阳性率无统计学差异,并且均能使细胞间质完全消化,细胞或重叠或孤立,细胞核中红绿信号清晰.结论 改良FISH法可以替代常规预处理步骤,简化了步骤,节省了时间,扩大了FISH检测试剂盒的临床应用.

Improvement in the detection of HER2 gene in breast cancer by fluorescence in situ hybridization at high altitude

Objective To improve and simplify the pretreatment procedure of fluorescent in situ hybridization （FISH） technique in the detection of HER2 gene in breast cancer tissues at high altitude, and to save the experiment time and promote the clinical application of FISH detection kit. Methods Ten breast cancer samples which had the HER2 immunohistochemistry staining results of ＋＋ were selected as the subjects. FISH kits from American PanPath company were used to change the pretreatment procedure into high-pressure boiling. Comparison was made in the effects of conventional and modified methods on the amplification of HER2 gene. Results There was no significant difference in the positive rate of HER2 gene detected by the two methods which both could make the intercellular substance digestion completely, the cells overlapped or isolated, and the red and green signal in the cell nucleus clear. Conclusion The modified FISH method can take the place of the common pretreatment, simplify the procedure, save the time, and promote the clinical application of the FISH detection kit.