| 氨茶碱、地塞米松对哮喘患者CD4+CD25+T调节细胞抑制功能的影响 |
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| 引用本文： | 张妍,周文英,邬伟明,黄瑾. 氨茶碱、地塞米松对哮喘患者CD4+CD25+T调节细胞抑制功能的影响[J]. 重庆医学, 2010, 39(1) |
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| 作者姓名： | 张妍 周文英 邬伟明 黄瑾 |
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| 作者单位： | 中山大学附属第五医院,呼吸科,广东珠海,519000;中山大学附属第五医院,中心实验室,广东珠海,519000 |
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| 摘 要： | 目的探讨哮喘患者CD4+ CD25+ T调节细胞的作用是否存在缺陷及药物(地塞米松、小剂量氨茶碱)对其作用的影响,为有效控制哮喘提供依据。方法分离哮喘患者及健康对照者外周血CD4+ CD25+ T调节细胞及CD4+CD25-T淋巴细胞并培养,设CD4+ CD25+ T调节细胞组、CD4+CD25-T淋巴细胞组及CD4+ CD25+ T调节细胞联合CD4+CD25-T淋巴细胞组,第3组又分为氨茶碱干预组、地塞米松干预组及空白对照组,72h后取培养上清液用ELISA法检测IFN-γ、IL-5、IL-13水平。结果健康对照者CD4+ CD25+ T调节细胞可抑制IFN-γ、IL-5及IL-13的生成(P<0.01),哮喘患者仅抑制IFN-γ、IL-5的生成(P<0.01);地塞米松预处理CD4+ CD25+ T调节细胞后,健康对照者CD4+ CD25+ T调节细胞抑制IFN-γ(P<0.05)、IL-5(P<0.01)、IL-13(P<0.01)生成能力增强,哮喘患者抑制IL-5、IL-13生成能力增强(P<0.01);采用氨茶碱预处理后,健康对照者CD4+ CD25+ T调节细胞抑制IL-5(P<0.01)、IL...
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| 关 键 词： | 支气管哮喘 CD4+CD25+T调节细胞 Th1/Th2细胞因子 地塞米松 氨茶碱 |
Effects of aminophylline and dexamethasone on inhibition of CD4+CD25+ regulatory T cells in asthmatic patients |
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| ZHANG Yan,ZHOU Wen-ying,WU Wei-ming,et al.. Effects of aminophylline and dexamethasone on inhibition of CD4+CD25+ regulatory T cells in asthmatic patients[J]. Chongqing Medical Journal, 2010, 39(1) |
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| Authors: | ZHANG Yan ZHOU Wen-ying WU Wei-ming et al. |
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| Affiliation: | 1.Department of Respiratory Medicine;2.Department of Central Laboratory;Fifth Affiliated Hospital of Sun Yat-sen University;Zhuhai;Guangdong 519000;China |
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| Abstract: | Objective To investigate whether the CD4+ CD25+ regulatory T cells in asthmatic patients act insufficiently and how dexamethasone and low dose of aminophylline affect the CD4+ CD25+ T cells.Methods CD4+ CD25+ and CD4+ CD25-T cells were separated from healthy controls and asthmatic patients,then cultured as CD4+ CD25+ T cell group,CD4+ CD25-T cell group and CD4+ CD25++CD4+ CD25+ T cell group,the last group was setted three sub-groups as dexamethasone group,aminophylline group and blank control.After 72h,the ... |
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| Keywords: | asthma CD4+ CD25+ regulatory T cell Th1/Th2 cytokines dexamethasone aminophylline |
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