大鼠输精管的神经分布及递质定位的探讨

A STUDY ON THE INNERVATION AND NEUROTRANSMITTERS LOCALIZATION IN THE RAT VAS DEFERENS

In this study gloxylic acid-induced fluorescent histochemical method was used to reveal the innervation of adrenergic nerves,acetycholinesterase (AChE) method to show the cholinergic nerves,and the immunohistochemical method to display the vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) immunoreactive nerves.This observation on the vas deferens showed that the VIP-immunoreactive nerves had the same distribution as the cholinergic nerves,and mainly existed in lamina propria,while NPY-immunoreactive nerves had the same distribution as adrenergic nerves and mainly existed in the muscular layer.One group of animals was treated by intraperitoneal injections of 6-OHDA on the lst,2nd,6th and 7th day,and killed on the 8th day.Following treatment with 6-OHDA,significant evanescence of most adrenergic nerves was observed.NPY-immunoreactive nerves reduced remarkably and the extent of the decrease was similiar to that of adrenergic nerves,whereas no change was observed on the VIP and cholinergic nerves.The immunoelectronic microscopy (PAP pre-embedding method) demonstrated that VIP immunoreactive complex deposited in the varicosity containing small clear vesicles (diameter of 40-55nm).Some varicosities also contained small number of large granular vesicles with the diameter of 100-144mm.NPY immunoreactive complex deposited in the large granular vesicles,and occasionally can be observed in the small granular vesicles,40-55nm in diameter.The results from this study suggest that VIP probably co-exist with ACh and NPY with noradrenalin in rat vas deferens.