| 芯片电泳快速检测高危型人类乳头状瘤病毒 |
| |
| 引用本文： | 陈斌,雷秀霞,汪安石,张佳宇,尹爱华,刘大渔,周小棉. 芯片电泳快速检测高危型人类乳头状瘤病毒[J]. 热带医学杂志, 2009, 9(11): 1227-1230 |
| |
| 作者姓名： | 陈斌 雷秀霞 汪安石 张佳宇 尹爱华 刘大渔 周小棉 |
| |
| 作者单位： | 广州医学院检验系,广州,510182;广州医学院附属广州市一人民医院检验科,广州,510180;广东省妇幼保健院,广州,510010 |
| |
| 基金项目： | 重大专项,国家自然科学基金,广州市科技攻关项目,广东省科技计划项目,广东省医学科学研究基金,广州市医药卫生基金 |
| |
| 摘 要： | 目的 建立人类乳头状瘤病毒(human papillomavims,HPV)52和58基因型高危型的芯片电泳检测方法.方法 选取114例宫颈细胞检测患者,提取宫颈脱落细胞DNA.PCR扩增HPV高危型52和58基因型特异性DNA序列,应用优化的芯片电泳分析方法对扩增产物进行分离检测.根据细胞学诊断结果将研究对象分为4组:正常组、不典型鳞状细胞组、低度鳞状上皮内病变组和高度鳞状上皮内病变组,比较各组的HPV52、58基因型感染情况.结果 114份标本中.HPV52基因型检出率为8.8%(10/114),HPV58基因型检出率为33.3%(38/114).与正常对照组相比较,各病变组HPV52基因型检出率差异有统计学意义(P<0.05);而HPV58基因型检出率在病变组和正常对照组之间差异无统计学意义(P>0.05).结论 芯片电泳法检测快速、成本低,可用于HPV52、58基因型感染的筛查.
|
| 关 键 词： | 芯片电泳 人乳头状瘤病毒 聚合酶链反应 |
Fast Detection of the High Risk Human Papillomaviruses Using Microchip Electrophoresis |
| |
| CHEN Bin,LEI Xiu-xia,WANG An-shi,ZHANG Jia-yu,YIN Ai-hua,LIU Da-yu,ZHOU Xiao-mian. Fast Detection of the High Risk Human Papillomaviruses Using Microchip Electrophoresis[J]. Journal Of Tropical Medicine, 2009, 9(11): 1227-1230 |
| |
| Authors: | CHEN Bin LEI Xiu-xia WANG An-shi ZHANG Jia-yu YIN Ai-hua LIU Da-yu ZHOU Xiao-mian |
| |
| Affiliation: | CHEN Bin1,LEI Xiu-xia2,WANG An-shi3,ZHANG Jia-yu3,YIN Ai-hua3,LIU Da-yu2,ZHOU Xiao-mian2 (1. Guangzhou Medical College,Guangzhou 510182,2. Department of Laboratory Medicine,Guangzhou First Municipal People's Hospital,Affiliated Hospital of Guangzhou Medical College,3. Guangdong Women and Children's Hospital and Health Institute,Guangzhou 510010,China) |
| |
| Abstract: | Objective A microchip electrophoresis based method was developed for the detection of high risk human papillomaviruses (HPV). Methods 114 cervical samples were classified into 4 groups according to their histological diagnosis as normal cytological diagnosis, unknown significance (ASCUS), low-grade intraepithelial lesions (LSIL), and high-grade intraepithelial lesions (HSIL). The extracted DNA samples were amplified with HPV 52 and 58 specific primers. The PCR amplicons were analyzed with an optimized micro... |
| |
| Keywords: | microchip electrophoresis human papillomavirus polymerase chain reaction |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
