高山红景天黄酮类化合物的分离与鉴定

目的对高山红景天药材的化学成分进行研究。方法采用反复硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱和半微量制备高效液相色谱分离纯化,根据ESI-MS、1H-NMR和13C-NMR等谱学数据进行结构鉴定。结果从高山红景天根和根茎的乙醇提取物中分离得到8个单体化合物,分别鉴定为黄酮类化合物herbacetin 3-O-[α-L-rhamnopyranosyl(1→2)-β-D-glucopyranosyl]-7-O-α-L-rhamnopyranoside(1)、三叶豆苷(trifolin,2)、草质素苷(rhodionin,3)、山奈酚(kaempferol,4)、山奈酚7-O-α-L-鼠李糖苷(kaempferol7-O-α-L-rhamnopyranoside,5)、小麦黄素(tricin,6)和大花红景天素(rhodiolinin,7);另外还得到2(3H)-苯并噻唑硫酮(2(3H)-benzothiazolethione,8)。结论化合物1为新化合物,化合物2和8为首次从景天科植物中分离得到。

Separation and identification of flavone compounds from root and rhizome of Rhodiola sachalinensis A.Bor.

Objective To study chemical constituents of root and rhizome of Rhodiola sachalinensis A. Bor. Methods The chemical constituents were isolated by silica, Sephadex LH-20, ODS column chromatography and HPLC. Chemical characters and spectroscopic analysis were employed for the structural identification. Results Eight compounds were obtained and their structures were identified as: herbacetin 3-O-[α-L-rhamnopyranosyl(1→2)-β-D-glucopyranosyl]-7-O-α-L-rhamnopyranoside (1), trifolin (2), rhodionin (3), kaempferol (4), kaempferol 7-O-α-L-rhamnopyranoside (5), tricin (6), rhodiolinin (7) and 2(3H)-benzothiazolethione (8). Conclusions Herbacetin 3-O- [α-L-rhamnopyranosyl (1→2)-β-D- glucopyranosyl]-7-O-α-L-rhamnopyranoside (1) is a new flavonol glucoside which has not been reported before. Compounds 2 and 8 are isolated from family Crassulaceae for the first time.