HPLC方法测定华佗再造丸中阿魏酸、吴茱萸内酯、吴茱萸碱和吴茱萸内碱的研究

目的 建立HPLC法测定华佗再造丸中阿魏酸、吴茱萸内酯、吴茱萸碱及吴茱萸次碱的含量.方法 采用Waters SunFire C18（4.6mm×250mm,5μm）色谱柱,以乙腈-0.1%磷酸进行梯度洗脱,流速1.0mL·min-1,阿魏酸检测波长为323nm,吴茱萸内酯、吴茱萸碱检测波长为225nm,吴茱萸次碱检测波长为343nm,柱温30℃.结果 表明阿魏酸、吴茱萸内酯、吴茱萸碱和吴茱萸次碱分别在3.91～39.10μg·mL-1（r=1.0000）、10.70～64.20μg·mL-1（r=0.9998）、7.60～76.00μg·mL-1（r=0.9999）、3.65～36.50μg·mL-1（r=0.9999）范围内与峰面积呈良好的线性关系;平均回收率（n=6）分别为96.6%（RSD=1.0%）、98.1%（RSD=2.2%）、100.3%（RSD=0.8%）、95.3%（RSD=1.2%）.结论 本文方法简便准确,可用于华佗再造丸中阿魏酸、吴茱萸内酯、吴茱萸碱及吴茱萸次碱的含量测定.

Analysis of Ferulic Acid,Evodin,Evodiamine and Rutaecarpine in Huatuo Zaizao Pills by HPLC

OBJECTIVE To establish an HPLC method for determination of ferulic acid, evodin, evodiamine and rutaecarpine in Huatuo zaizao pills. METHODS The separation was achieved on a Waters SunFire C18 （4. 6mm ×250mm,5μm） column with the gradient elution of acetonitrile-0. 1% phosphoric acid solution at a flow rate of 1.0mL ·min^-1. The UV detection wavelength was set at 323nm for ferulic acid,225nm for evodin and evodiamine, and 343nm for rutaecarpine. The column temperature was 30℃. RESULTS The linear ranges of ferulic acid,evodin,evodiamine and rutaecarpine were 3.91 - 39. 10μg·mL^-1 （ r = 1. 0000）, 10. 70 - 64. 20μg·mL^-1（ r = 0. 9998 ） ,7.60 - 76. 00μg·mL^-1 （ r = 0. 9999 ）, 3.65 - 36. 50μg·mL^-1（ r = 0. 9999 ）, respectively. The average recoveries （n =6） were 96.6% （RSD = 1.0% ） ,98.1% （RSD =2. 2% ） ,100. 3% （RSD =0. 8% ） ,95. 3% （RSD = 1.2% ）, respectively. CONCLUSION The method is simple and aecurate, which can be successfully applied to the analysis of ferulic acid, evodin, evodiamine and rutaeearpine in Huatuo zaizao pills.